Planta

, Volume 226, Issue 6, pp 1491–1499

Diurnal regulation of scent emission in rose flowers

  • Keren Hendel-Rahmanim
  • Tania Masci
  • Alexander Vainstein
  • David Weiss
Original Article

DOI: 10.1007/s00425-007-0582-3

Cite this article as:
Hendel-Rahmanim, K., Masci, T., Vainstein, A. et al. Planta (2007) 226: 1491. doi:10.1007/s00425-007-0582-3

Abstract

Previous studies have shown diurnal oscillation of scent emission in rose flowers with a peak during the day (Helsper in Planta 207:88–95, 1998; Picone in Planta 219:468–478, 2004). Here, we studied the regulation of scent production and emission in Rosa hybrida cv. Fragrant Cloud during the daily cycle and focused on two terpenoid compounds, germacrene D and geranyl acetate, whose biosynthetic genes have been characterized by us previously. The emission of geranyl acetate oscillated during the daily light/dark cycle with a peak early in the light period. A similar daily fluctuation was found in the endogenous level of this compound and in the expression of its biosynthetic gene, alcohol acetyl transferase (RhAAT). The rhythmic expression of RhAAT continued under conditions of constant light or darkness, indicating regulation by the endogenous circadian clock. However, the accumulation and emission of geranyl acetate ceased under continuous light. Our results suggest that geranyl acetate production is limited by the level of its substrate geraniol, which is suppressed under constant light conditions. The emission of germacrene D also oscillated during the daily cycle with a peak early in the light period. However, the endogenous level of this compound and the expression of its biosynthetic gene germacrene D synthase (RhGDS) were constant throughout the day. The diurnal oscillation of germacrene D emission ceased under continuous light, suggesting direct regulation by light. Our results demonstrate the complexity of the diurnal regulation of scent emission: although the daily emission of most scent compounds is synchronized, various independently evolved mechanisms control the production, accumulation and release of different volatiles.

Keywords

Circadian rhythm Floral scent Geranyl acetate Germacrene D Rose 

Abbreviations

2-PE

2-Phenylethanol

2-PEG

Glycosylated 2-PE;

BAMT

Benzoic acid carboxyl methyltransferase

RhAAT

Rosa hybrida alcohol acetyl transferase

RhGDS

Rosa hybrida germacrene D synthase

SAMT

S-Adenosyl-l-Met:salicylic acid carboxyl methyltransferase

Supplementary material

425_2007_582_MOESM1_ESM.doc (54 kb)
(DOC 53 kb)
425_2007_582_MOESM2_ESM.doc (46 kb)
(DOC 45 kb)

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Keren Hendel-Rahmanim
    • 1
  • Tania Masci
    • 1
  • Alexander Vainstein
    • 1
  • David Weiss
    • 1
  1. 1.Faculty of Agricultural, Food and Environmental Quality Sciences, The Robert H. Smith Institute of Plant Sciences and Genetics in AgricultureThe Hebrew University of JerusalemRehovotIsrael

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