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Planta

, Volume 226, Issue 4, pp 867–876 | Cite as

Characterization of 1-aminocyclopropane-1-carboxylate (ACC) deaminase containing Methylobacterium oryzae and interactions with auxins and ACC regulation of ethylene in canola (Brassica campestris)

  • Munusamy Madhaiyan
  • Selvaraj Poonguzhali
  • Tongmin SaEmail author
Original Article

Abstract

The possible interaction of the plant hormones auxin and ethylene and the role of 1-aminocyclopropane-1-carboxylate (ACC) deaminase containing bacteria on ethylene production in canola (Brassica campestris) in the presence of inhibitory concentrations of growth regulators were investigated. The effects of auxin (indole-3-acetic acid and 2,4-dichlorophenoxy acetic acid), auxin transport inhibitor 2-(p-chlorophenoxy)-2-methylpropionic acid, ethylene precursor 1-aminocyclopropane-1-carboxylate and ethylene synthesis inhibitor l-α-(2-aminoethoxyvinyl)glycine hydrochloride on root elongation were concentration dependent. Exogenous addition of growth regulators influences the enzyme activities of ethylene production and we have presented here evidences that support the hypothesis that inhibitory effects of auxin on root elongation are independent of ethylene. Additionally, we have proved that inoculation of ACC deaminase containing Methylobacterium oryzae sequester ACC exuded from roots and hydrolyze them lowering the concentration of ACC in root exudates. However, the inhibitory actions of exogenous additions of auxins could not be ameliorated by bacterial inoculation that reduces ethylene concentration in canola seedlings.

Keywords

ACC deaminase Auxin Ethylene Methylobacterium Root growth Canola 

Abbreviations

2,4-D

2,4-Dichlorophenoxy acetic acid

ACC

1-Aminocyclopropane-1-carboxylate

ACO

ACC oxidase

ACS

ACC synthase

ACCd

ACC deaminase

AMS

Ammonium mineral salts

AVG

l-α-(2-aminoethoxyvinyl)glycine hydrochloride

CMPA

2-(p-chlorophenoxy)-2-methylpropionic acid

IAA

Indole-3-acetic acid

PGPB

Plant growth promoting bacteria

SAM

S-adenosyl-l-methionine

Notes

Acknowledgments

This work was supported in part by the funds from Rural Development Administration (RDA), Republic of Korea. S. Poonguzhali and M. Madhaiyan are supported by the grants from the Korea Research Foundation (KRF), under the fellowship for Ph.D students and through the Program for Foreign Scientist and Engineers for which we are grateful.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Munusamy Madhaiyan
    • 1
  • Selvaraj Poonguzhali
    • 1
  • Tongmin Sa
    • 1
    Email author
  1. 1.Department of Agricultural ChemistryChungbuk National UniversityChungbukRepublic of Korea

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