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Planta

, 226:839 | Cite as

Rhizobacterial volatile emissions regulate auxin homeostasis and cell expansion in Arabidopsis

  • Huiming Zhang
  • Mi-Seong Kim
  • Venkat Krishnamachari
  • Paxton Payton
  • Yan Sun
  • Mark Grimson
  • Mohamed A. Farag
  • Choong-Min Ryu
  • Randy Allen
  • Itamar S. Melo
  • Paul W. ParéEmail author
Original Article

Abstract

Certain plant growth-promoting rhizobacteria (PGPR), in the absence of physical contact with a plant stimulate growth via volatile organic compound (VOC) emissions, through largely unknown mechanisms. To probe how PGPR VOCs trigger growth in plants, RNA transcript levels of Arabidopsis seedlings exposed to Bacillus subtilus (strain GB03) were examined using oligonucleotide microarrays. In screening over 26,000 protein-coded transcripts, a group of approximately 600 differentially expressed genes related to cell wall modifications, primary and secondary metabolism, stress responses, hormone regulation and other expressed proteins were identified. Transcriptional and histochemical data indicate that VOCs from the PGPR strain GB03 trigger growth promotion in Arabidopsis by regulating auxin homeostasis. Specifically, gene expression for auxin synthesis was up regulated in aerial regions of GB03-exposed plants; auxin accumulation decreased in leaves and increased in roots with GB03 exposure as revealed in a transgenic DR5::GUS Arabidopsis line, suggesting activation of basipetal auxin transport. Application of the auxin transport inhibitor 1-naphthylphthalamic acid (NPA) restricted auxin accumulation to sites of synthesis thereby preventing GB03-mediated decreases in shoot auxin levels as well as thwarting GB03-mediated growth promotion. In addition, microarray data revealed coordinated regulation of cell wall loosening enzymes that implicated cell expansion with GB03 exposure, which was confirmed by comparative cytological measurements. The discovery that bacterial VOCs, devoid of auxin or other known plant hormones regulate auxin homeostasis and cell expansion provides a new paradigm as to how rhizobacteria promote plant growth.

Keywords

Auxin transport Bacillus subtilus GB03 Cell expansion Plant growth promotion Transcriptional profiling Rhizobacterial signaling 

Abbreviations

IAA

Indole acetic acid

NPA

1-Naphthylphthalamic acid

PGPR

Plant growth promotion rhizobacteria

rt-PCR

Real time PCR

RT-PCR

Reverse transcriptase PCR

VOCs

Volatile organic compounds

Notes

Acknowledgments

We especially want to thank Drs. Rangasamy Elumalai for technical expertise with microarray analyses; Mary Catherine Hastert for technical expertise with microscopic analyses; and Tom Guilfoyle for the DR5::GUS line. This research was funded in part by the Welch Foundation (Grant D1478), The Frasch Foundation for Chemical Research, Biogreen 21 and Technology Development Programs for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

Supplementary material

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Huiming Zhang
    • 1
  • Mi-Seong Kim
    • 1
  • Venkat Krishnamachari
    • 1
  • Paxton Payton
    • 2
  • Yan Sun
    • 1
  • Mark Grimson
    • 1
  • Mohamed A. Farag
    • 1
  • Choong-Min Ryu
    • 3
  • Randy Allen
    • 1
  • Itamar S. Melo
    • 4
  • Paul W. Paré
    • 1
    Email author
  1. 1.Departments of Chemistry/Biochemistry and BiologyTexas Tech UniversityLubbockUSA
  2. 2.USDA-ARS Cropping Systems LabLubbockUSA
  3. 3.Microbial Genomics LabKorean Research Institute of Biosci/Biotech, YusongTaejonSouth Korea
  4. 4.EMBRAPA Meio AmbienteJaguariúnaBrazil

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