Planta

, Volume 226, Issue 4, pp 815–825

ERF protein JERF1 that transcriptionally modulates the expression of abscisic acid biosynthesis-related gene enhances the tolerance under salinity and cold in tobacco

  • Lijun Wu
  • Xiaoliang Chen
  • Haiyun Ren
  • Zhijin Zhang
  • Haiwen Zhang
  • Junying Wang
  • Xue-Chen Wang
  • Rongfeng Huang
Original Article

DOI: 10.1007/s00425-007-0528-9

Cite this article as:
Wu, L., Chen, X., Ren, H. et al. Planta (2007) 226: 815. doi:10.1007/s00425-007-0528-9

Abstract

Increasing evidences indicate that ethylene responsive factor (ERF) proteins regulate a variety of biotic and abiotic stress responses, and plant development as well. Previously we demonstrated that JERF1, encoding an ERF transcriptional activator, is inducible by ethylene, MeJA, ABA, and NaCl, suggesting its possible regulation in multiple stress responses. In the present paper, we report that expressing JERF1 in tobacco increases the seed germination under mannitol treatment, and enhances the tolerance to high salinity and low temperature, through accumulating sodium in vacuole of leaves and stabilizing the plasma membrane, respectively, and significantly increases the growth of tobacco roots and leaves under salinity and low temperature through an unknown mechanism. The evidence that JERF1 interacts with multiple cis-acting elements, such as GCC-box, DRE, and CE1, to activate the expression of stress-related genes, supports the possible involvement of JERF1 in multiple plant stress responses with ABA-dependent and ABA-independent manner. More importantly, we reveal that expressing JERF1 in tobacco transcriptionally regulates the expression of ABA biosynthesis-related gene NtSDR, resulting in the increase of the ABA content. Together, our results indicate that JERF1 interacts with multiple cis-acting elements and activates the expression of stress responsive and ABA biosynthesis-related genes, consequently causing ABA biosynthesis, and ultimately enhancing tobacco tolerance and growth under high salinity and low temperature.

Keywords

Abscisic acid High salt Low temperature Multiple stresses Transcriptional activator JERF1 

Abbreviations

ABA

Abscisic acid

ABRE

ABA responsive element

CaMV

Cauliflower mosaic virus

CE1

Coupled element 1

DRE/CRT

Dehydration-responsive element/C-repeat

ERF

Ethylene-responsive factor

JERE

JA- and elicitor-responsive element

MeJA

Methyl jasmonate

OE

Overexpression of JERF1 in tobacco

PR

Pathogenesis-related

TAIL-PCR

Thermal asymmetric interlaced PCR

WT

Wild type

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Lijun Wu
    • 1
    • 2
    • 3
    • 4
  • Xiaoliang Chen
    • 2
    • 3
    • 4
    • 5
  • Haiyun Ren
    • 5
  • Zhijin Zhang
    • 1
    • 2
    • 3
    • 4
  • Haiwen Zhang
    • 2
    • 3
    • 4
  • Junying Wang
    • 2
    • 3
    • 4
  • Xue-Chen Wang
    • 1
  • Rongfeng Huang
    • 2
    • 3
    • 4
  1. 1.National Laboratory of Plant Physiology and Biochemistry, College of Biological SciencesChina Agricultural UniversityBeijingChina
  2. 2.Biotechnology Research InstituteChinese Academy of Agricultural SciencesBeijingChina
  3. 3.National Key Facility of Crop Gene Resources and Genetic ImprovementBeijingChina
  4. 4.National Center for Plant Gene ResearchBeijingChina
  5. 5.College of Life SciencesBeijing Normal UniversityBeijingChina

Personalised recommendations