Developmental control of Arabidopsis seed oil biosynthesis
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Arabidopsis transcriptional factors LEAFY COTYLEDON1 (LEC1), LEAFY COTYLEDON2 (LEC2), FUSCA3 (FUS3), ABSCISIC ACID3 (ABI3), and ABSCISIC ACID5 (ABI5) are known to regulate multiple aspects of seed development. In an attempt to understand the developmental control of storage product accumulation, we observed the expression time course of the five transcripts. The sequential expression of these factors during seed fill suggests differentiation of their normal responsibilities. By extending the expression periods of the two early genes LEC1 and LEC2 in transgenic seeds, we demonstrated that the subsequent timing of FUS3, ABI3, and ABI5 transcripts depends on LEC1 and LEC2. Because a delayed onset or reduced level of FUS3 mRNA coincided with reduction of seed oil content in the transgenic seeds, the role of FUS3 in oil deposition was further examined. Analysis of published seed transcriptome data indicated that FUS3 transcript increased together with nearly all the plastidial fatty acid biosynthetic transcripts during development. The ability of FUS3 to rapidly induce fatty acid biosynthetic gene expression was confirmed using transgenic Arabidopsis seedlings expressing a dexamethasone (DEX)-inducible FUS3 and Arabidopsis mesophyll protoplasts transiently expressing the FUS3 gene. By accommodating the current evidence, we propose a hierarchical architecture of the transcriptional network in Arabidopsis seeds in which the oil biosynthetic pathway is integrated through the master transcriptional factor FUS3.
KeywordsSeed Oil Fatty acid Regulation of gene expression Microarray
ABSCISIC ACID INSENSITIVE3
ABSCISIC ACID INSENSITIVE5
Day after fertilization
We thank Dr. Mary Schuler for critical reading of the manuscript, Drs. Don Ort, Steve Clough, and Matt Hudson for sharing equipment. This work was supported by funds from Department of Crop Sciences and University of Illinois.
- Mansfield SG, Briarty LG (1992) Cotyledon cell development in Arabidopsis thaliana during reserve deposition. Can J Bot 70:151–164Google Scholar
- Nambara E, Keith K, McCourt P, Naito S (1995) A regulatory role for the ABI3 gene in the establishment of embryo maturation in Arabidopsis thaliana. Development 121:629–636Google Scholar