, Volume 226, Issue 3, pp 797–803

Tissue integrity and RNA quality of laser microdissected phloem of potato

Rapid Communication


The phloem is an important conduit for the transport of signaling molecules including RNA. Phloem sap has served as a source of RNA to profile uncontaminated phloem transcriptomes but its collection is difficult in many species. Laser capture microdissection techniques offer a valuable alternative for isolating RNA from specific vascular cells. In potato (Solanum tuberosum L.), there are seven BEL1-like transcription factors expressed throughout the plant with diverse functions. The RNA of one of these, StBEL5, moves through the phloem from the leaf to stolon tips to regulate tuber formation. In this study, the presence of several BEL RNAs and one Knotted1-like RNA was determined in phloem cells collected by laser microdissection coupled to laser pressure catapulting (LMPC). Three fixatives were compared for their effect on cell morphology and RNA quality in transverse sections of stems of potato. For optimum tissue integrity and quality of RNA from potato stem sections, the best results were achieved using ethanol acetate as the fixative. In addition, the RT-PCR results demonstrated the presence of six out of seven of the StBEL RNAs and a potato Knox RNA in phloem cells.


Microdissection Phloem RNA Plant stem Tuberization Vascular tissue 



Base pairs


Laser capture microdissection


Laser microdissection coupled to laser pressure catapulting


Laser pressure catapulting

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • YueYue Yu
    • 1
    • 2
    • 3
  • C. C. Lashbrook
    • 3
  • David J. Hannapel
    • 1
    • 3
  1. 1.Molecular, Cellular, and Developmental Biology MajorIowa State UniversityAmesUSA
  2. 2.Department of Plant PathologyIowa State UniversityAmesUSA
  3. 3.Department of HorticultureIowa State UniversityAmesUSA

Personalised recommendations