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Planta

, Volume 226, Issue 2, pp 465–474 | Cite as

Nitrite-driven anaerobic ATP synthesis in barley and rice root mitochondria

  • Maria Stoimenova
  • Abir U. Igamberdiev
  • Kapuganti Jagadis Gupta
  • Robert D. Hill
Original Article

Abstract

Mitochondria isolated from the roots of barley (Hordeum vulgare L.) and rice (Oryza sativa L.) seedlings were capable of oxidizing external NADH and NADPH anaerobically in the presence of nitrite. The reaction was linked to ATP synthesis and nitric oxide (NO) was a measurable product. The rates of NADH and NADPH oxidation were in the range of 12–16 nmol min−1 mg−1 protein for both species. The anaerobic ATP synthesis rate was 7–9 nmol min−1 mg−1 protein for barley and 15–17 nmol min−1 mg−1 protein for rice. The rates are of the same order of magnitude as glycolytic ATP production during anoxia and about 3–5% of the aerobic mitochondrial ATP synthesis rate. NADH/NADPH oxidation and ATP synthesis were sensitive to the mitochondrial inhibitors myxothiazol, oligomycin, diphenyleneiodonium and insensitive to rotenone and antimycin A. The uncoupler FCCP completely eliminated ATP production. Succinate was also capable of driving ATP synthesis. We conclude that plant mitochondria, under anaerobic conditions, have a capacity to use nitrite as an electron acceptor to oxidize cytosolic NADH/NADPH and generate ATP.

Keywords

Anaerobiosis Mitochondria Hordeum vulgare Nitric oxide Nitrite Oryza sativa 

Abbreviations

AOX

Alternative oxidase

COX

Cytochrome c oxidase

DPI

Diphenyleneiodonium

ETC

Electron transport chain

FCCP

Carbonylcyanide-p-trifluoromethoxyphenylhydrazone

Hb

Hemoglobin

Notes

Acknowledgments

We thank Werner M. Kaiser for his cooperation and helpful suggestions. This work was supported by the Natural Sciences and Engineering Research Council of Canada and Genome Canada (to R.D.H.).

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Maria Stoimenova
    • 1
  • Abir U. Igamberdiev
    • 1
  • Kapuganti Jagadis Gupta
    • 2
  • Robert D. Hill
    • 1
  1. 1.Department of Plant ScienceUniversity of ManitobaWinnipegCanada
  2. 2.Julius-von-Sachs-Institut fur BiowissenschaftenWürzburgGermany

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