Planta

, Volume 225, Issue 6, pp 1339–1351 | Cite as

AtGRP2, a cold-induced nucleo-cytoplasmic RNA-binding protein, has a role in flower and seed development

  • Adriana Flores Fusaro
  • Silvia Nora Bocca
  • Rose Lucia Braz Ramos
  • Rosa Maria Barrôco
  • Claudia Magioli
  • Vanessa Cardeal Jorge
  • Tatiana Cardoso Coutinho
  • Camila Martins Rangel-Lima
  • Riet De Rycke
  • Dirk Inzé
  • Gilbert Engler
  • Gilberto Sachetto-Martins
Original Article

Abstract

The glycine-rich protein AtGRP2 is one of the four members of the cold-shock domain (CSD) protein family in Arabidopsis. It is characterized by the presence of a nucleic acid-binding CSD domain, two glycine-rich domains and two CCHC zinc-fingers present in nucleic acid-binding proteins. In an attempt to further understand the role of CSD/GRP proteins in plants, we have proceeded to the functional characterization of the AtGRP2 gene. Here, we demonstrate that AtGRP2 is a nucleo-cytoplasmic protein involved in Arabidopsis development with a possible function in cold-response. Expression analysis revealed that the AtGRP2 gene is active in meristematic tissues, being modulated during flower development. Down-regulation of AtGRP2 gene, using gene-silencing techniques resulted in early flowering, altered stamen number and affected seed development. A possible role of AtGRP2 as an RNA chaperone is discussed.

Keywords

Arabidopsis Cold-shock protein Development Flowering time Glycine-rich protein; RNA-binding protein 

Abbreviations:

CDS

Cold-shock domain

CSP

Cold-shock protein

CCHC

Knuckle zinc finger

GRP

Glycine-rich protein

GUS

β-glucuronidase

MBP

Maltose-binding protein

RRM

RNA recognition motif

Notes

Acknowledgments

The authors would like to thank D. E. de Oliveira (Institute of Plant Biotechnology for Developing Countries, Gent, Belgium) for critical reading of the manuscript. A.F.F. was supported by a Ph.D. fellowship from CAPES. S.N.B. and C.M. were supported by CNPq post-doctoral and CAPES-ProDoc fellowships, respectively. V.C.J., T.C.C. and C.M.R.L. were recipient of PIBIC fellowship from CNPq. This work was supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Fundação Carlos Chagas de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) to G.S.M. and by Interuniversity Poles of Attraction Programme-Belgian Science Policy (P5/13) to D.I.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Adriana Flores Fusaro
    • 1
  • Silvia Nora Bocca
    • 1
  • Rose Lucia Braz Ramos
    • 1
  • Rosa Maria Barrôco
    • 2
  • Claudia Magioli
    • 1
  • Vanessa Cardeal Jorge
    • 1
  • Tatiana Cardoso Coutinho
    • 1
  • Camila Martins Rangel-Lima
    • 1
  • Riet De Rycke
    • 2
  • Dirk Inzé
    • 2
  • Gilbert Engler
    • 3
    • 4
  • Gilberto Sachetto-Martins
    • 1
  1. 1.Laboratório de Genética Molecular Vegetal, Departamento de GenéticaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB)Ghent UniversityGentBelgium
  3. 3.Laboratoire Associé de l’Institut National de la Recherche Agronomique (France)Universiteit GentGentBelgium
  4. 4.Department for Plant Health and the Environment,Institut National de la Recherche AgronomiqueAntibesFrance

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