Planta

, Volume 224, Issue 6, pp 1495–1502 | Cite as

Evidence for intracellular spatial separation of hexokinases and fructokinases in tomato plants

  • Hila Damari-Weissler
  • Michal Kandel-Kfir
  • David Gidoni
  • Anahit Mett
  • Eddy Belausov
  • David Granot
Rapid Communication

Abstract

Four hexokinase (LeHXK1–4) and four fructokinase (LeFRK1–4) genes were identified in tomato plants. Previous GFP fusion studies indicate that the gene product of LeHXK3 is associated with the mitochondria while that of LeHXK4 is located within plastids. In this study we found that the enzyme encoded by the fructokinase gene LeFRK3 is also located within plastids. The presence of LeFrk3 enzyme in plastids raises the question of the origin of fructose in these organelles. The other three FRKs enzymes, LeFrk1&2&4, are located in the cytosol. Unlike LeFrk1&2&4, the two additional HXKs, LeHxk1&2, share a common membrane anchor domain and are associated with the mitochondria similar to LeHxk3. The difference in the locations of the cytoplasmic FRK and HXK isozymes suggests that glucose phosphorylation is confined to defined special intracellular localizations while fructose phosphorylation is less confined.

Keywords

Fructokinase GFP Hexokinase Hexose phosphorylation Intracellular localization Tomato (Lycopersicon esculentum

Abbreviations

FRK

Fructokinase

GFP

Green fluorescent protein

HXK

Hexokinase

Notes

Acknowledgments

This research was supported by research grant No. 582/01 from The Israel Science Foundation, and by research grant No. IS-3326-02C from BARD, the United States—Israel Binational Agricultural and Development Fund.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Hila Damari-Weissler
    • 1
  • Michal Kandel-Kfir
    • 1
  • David Gidoni
    • 1
  • Anahit Mett
    • 1
  • Eddy Belausov
    • 1
  • David Granot
    • 1
  1. 1.Institute of Plant Sciences, Agricultural Research OrganizationThe Volcani CenterBet DaganIsrael

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