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Planta

, Volume 225, Issue 2, pp 287–300 | Cite as

The promoter of the wheat puroindoline-a gene (PinA) exhibits a more complex pattern of activity than that of the PinB gene and is induced by wounding and pathogen attack in rice

  • Alexandre Evrard
  • Donaldo Meynard
  • Emmanuel Guiderdoni
  • Philippe Joudrier
  • Marie-Françoise Gautier
Original Article

Abstract

Puroindolines form the molecular basis of wheat grain hardness. However, little is known about puroindoline gene regulation. We previously reported that the Triticum aestivum puroindoline-b gene (PinB) promoter directs β-glucuronidase gene (uidA) seed-specific expression in transgenic rice. In this study, we isolated a puroindoline-a gene (PinA), analyzed PinA promoter activity by 5′ deletions and compared PinA and PinB promoters in transgenic rice. Seeds of PinA-1214 and PinB-1063 transgenic plants strongly expressed uidA in endosperm, in the aleurone layer and in epidermis cells in a developmentally regulated manner. The GUS activity was also observed in PinA-1214 embryos. Whereas the PinB promoter is seed specific, the PinA promoter also directed, but to a lower level, uidA expression in roots of seedlings and in the vascular tissues of palea and pollen grains of dehiscent anthers during flower development. In addition, the PinA promoter was induced by wounding and by Magnaporthe grisea. By deletion analysis, we showed that the “390-bp” PinA promoter drives the same expression pattern as the “1214-bp” promoter. Moreover, the “214-bp” PinA promoter drives uidA expression solely in pollen grains of dehiscent anthers. The presence of putative cis-regulatory elements that may be related to PinA expression is discussed from an evolutionary point of view. By electrophoretic mobility shift assay, we showed that putative cis-elements (WUN-box, TCA motifs and as-1-like binding sites) whose presence in the PinA promoter may be related to wounding and/or the pathogen response form complexes with nuclear extracts isolated from wounded wheat leaves.

Keywords

Plant defense Promoter analysis Puroindoline-gene regulation Transgenic rice uidA-gene reporter Wheat 

Abbreviations

DAF

Days after flowering

DAG

Days after germination

EMSA

Electrophoretic mobility shift assay

GUS

β-Glucuronidase

hfw

Hours following wounding

hpi

Hours post-inoculation

MATAB

Mixed alkyl trimethyl ammonium bromide

PinA

Triticum aestivum puroindoline-a gene

PIN-a

Puroindoline-a

PinB

T. aestivum puroindoline-b gene

PIN-b

Puroindoline-b

PCR

Polymerase chain reaction

UidA

β-Glucuronidase gene

UTR

Untranslated region

Notes

Acknowledgements

The authors wish to thank Dr. Didier Tharreau for providing the FR13 isolate and inoculation protocol. We would also like to thank Jacques Escoute for his technical help and advice on histological analysis. Alexandre Evrard was the recipient of a fellowship from the French Ministère de l’Education Nationale, de l’Enseignement Supérieur et de la Recherche. The support of the Génopole LR for containment greenhouse infrastructures is also acknowledged.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Alexandre Evrard
    • 1
    • 3
  • Donaldo Meynard
    • 2
  • Emmanuel Guiderdoni
    • 2
  • Philippe Joudrier
    • 1
  • Marie-Françoise Gautier
    • 1
  1. 1.INRA, UMR1096 PIAMontpellier Cedex 01France
  2. 2.Cirad Amis, UMR 1096, PIA, TA40/03Montpellier Cedex 05France
  3. 3.Department of Botany and Plant SciencesUniversity of CaliforniaRiversideUSA

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