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Planta

, Volume 225, Issue 1, pp 193–202 | Cite as

Cellular and subcellular compartmentation of Ni in the Eurasian serpentine plants Alyssum bracteatum, Alyssum murale (Brassicaceae) and Cleome heratensis (Capparaceae)

  • T. Asemaneh
  • S. M. Ghaderian
  • S. A. Crawford
  • A. T. Marshall
  • A. J. M. BakerEmail author
Original Paper

Abstract

This study investigated the cellular and subcellular compartmentation of Ni in the Eurasian serpentine species Alyssum murale, Alyssum bracteatum and Cleome heratensis and a non-serpentine population of A. murale (as a control) grown in hydroponic culture. Plant growth responses and Ni uptake clearly revealed the higher Ni tolerance of serpentine plants than the non-serpentine plants. Serpentine A. murale and A. bracteatum grew better at elevated (0.01 mM) Ni in the nutrient solution, supporting the view that the Ni hyperaccumulators have a higher requirement for Ni than normal plants. Low shoot Ni content of C. heratensis in response to the high Ni treatments indicated that this species employs an avoidance strategy for Ni tolerance. Energy-dispersive X-ray microanalysis showed that Ni was highly concentrated in the cell walls and cell lumen, most likely the vacuoles, of leaf epidermis of A. murale and A. bracteatum rather than in the mesophyll cells. EDX spectra from leaves of the non-serpentine A. murale suggested that Ni accumulated in both epidermal and mesophyll cells but not in the epidermal cell walls. Growth reduction and Ni toxicity in plants of the non-serpentine A. murale could be due to accumulation of Ni in the lumen of leaf mesophyll cells. Our data suggest that cellular and subcellular compartmentation are both possible mechanisms for Ni tolerance employed by the serpentine A. murale and A. bracteatum.

Keywords

Alyssum Cleome Nickel (tolerance uptake) Nickel localization Hyperaccumulator 

Abbreviations

EDX

Energy-dispersive X-ray microanalysis

STEM

Scanning transmission electron microscope

Notes

Acknowledgments

A scholarship from the Ministry of Science, Research and Technology of Iran (MSRT), Yasuj University and Isfahan University to TA is gratefully acknowledged. We are very grateful to Dr R. Glaisher (La Trobe University) for assistance with EDX microanalyses and elemental mapping. We also thank the School of Botany (University of Melbourne) for providing research facilities.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • T. Asemaneh
    • 1
    • 2
  • S. M. Ghaderian
    • 1
  • S. A. Crawford
    • 2
  • A. T. Marshall
    • 3
  • A. J. M. Baker
    • 2
    Email author
  1. 1.Department of BiologyUniversity of IsfahanIsfahanIran
  2. 2.School of BotanyUniversity of MelbourneParkvilleAustralia
  3. 3.School of ZoologyLa Trobe UniversityBundooraAustralia

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