, Volume 225, Issue 1, pp 153–164 | Cite as

Genetic and transgenic perturbations of carbon reserve production in Arabidopsis seeds reveal metabolic interactions of biochemical pathways

  • Yun Lin
  • Alexander V. Ulanov
  • Vera Lozovaya
  • Jack Widholm
  • Guirong Zhang
  • Jinhua Guo
  • Howard M. Goodman
Original Article


The biosynthesis of seed oil and starch both depend on the supply of carbon from the maternal plant. The biochemical interactions between these two pathways are not fully understood. In the Arabidopsis mutant shrunken seed 1 (sse1)/pex16, a reduced rate of fatty acid synthesis leads to starch accumulation. To further understand the metabolic impact of the decrease in oil synthesis, we compared soluble metabolites in sse1 and wild type (WT) seeds. Sugars, sugar phosphates, alcohols, pyruvate, and many other organic acids accumulated in sse1 seeds as a likely consequence of the reduced carbon demand for lipid synthesis. The enlarged pool size of hexose-P, the metabolites at the crossroad of sugar metabolism, glycolysis, and starch synthesis, was likely a direct cause of the increased flow into starch. Downstream of glycolysis, more carbon entered the TCA cycle as an alternative to the fatty acid pathway, causing the total amount of TCA cycle intermediates to rise while moving the steady state of the cycle away from fumarate. To convert the excess carbon metabolites into starch, we introduced the Escherichia coli starch synthetic enzyme ADP-glucose pyrophosphorylase (AGPase) into sse1 seeds. Expression of AGPase enhanced net starch biosynthesis in the mutant, resulting in starch levels that reached 37% of seed weight. However, further increases above this level were not achieved and most of the carbon intermediates remained high in comparison with the WT, indicating that additional mechanisms limit starch deposition in Arabidopsis seeds.


Seeds Storage Metabolite profiling Carbon metabolism Oil Starch Arabidopsis thaliana 



We thank Dr. Nicki Engeseth for critical reading of the manuscript. This work was supported by start-up funds from the Department of Crop Sciences, University of Illinois to Y.L., The Illinois Agricultural Experimental Station, and a grant from The Department of Molecular Biology, Massachusetts General Hospital to H.M.G.

Supplementary material

425_2006_337_MOESM1_ESM.doc (38 kb)
Supplementary material


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Yun Lin
    • 1
  • Alexander V. Ulanov
    • 1
  • Vera Lozovaya
    • 1
  • Jack Widholm
    • 1
  • Guirong Zhang
    • 1
  • Jinhua Guo
    • 1
  • Howard M. Goodman
    • 2
    • 3
  1. 1.Department of Crop SciencesUniversity of IllinoisUrbanaUSA
  2. 2.Department of Molecular BiologyMassachusetts General HospitalBostonUSA
  3. 3.Department of GeneticsHarvard Medical SchoolBostonUSA

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