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Planta

, Volume 224, Issue 6, pp 1485–1494 | Cite as

Hypergravity induces reorientation of cortical microtubules and modifies growth anisotropy in azuki bean epicotyls

  • Kouichi SogaEmail author
  • Kazuyuki Wakabayashi
  • Seiichiro Kamisaka
  • Takayuki Hoson
Original Article

Abstract

We examined the changes in the orientation of cortical microtubules during the hypergravity-induced modification of growth anisotropy (inhibition of elongation growth and promotion of lateral growth) in azuki bean (Vigna angularis Ohwi et Ohashi) epicotyls. The percentage of cells with transverse microtubules was decreased, while that with longitudinal microtubules was increased, in proportion to the logarithm of the magnitude of gravity. The percentage of cells with longitudinal microtubules showed an increase within 0.5 h of transfer of the 1g-grown seedlings to a 300g-hypergravity condition. Lanthanum and gadolinium, blockers of calcium channels, nullified the modification of growth anisotropy and reorientation of microtubules by hypergravity. Horizontal and acropetal hypergravity modified growth anisotropy and reorientation of microtubules, as did basipetal hypergravity, and these changes were not seen in the presence of lanthanum or gadolinium. These results suggest that hypergravity changes activities of lanthanum- and gadolinium-sensitive calcium channels independently of its direction, which may lead to reorientation of cortical microtubules and modification of growth anisotropy in azuki bean epicotyls.

Keywords

Azuki bean (Vigna angularis Ohwi et Ohashi) Epicotyl Growth anisotropy Hypergravity Microtubule 

Abbreviations

PBS

Phosphate-buffered saline

Notes

Acknowledgments

The present study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, a Grant for Ground-based Research for Space Utilization from Japan Space Forum, and by Sasakawa Scientific Research Grant from the Japan Science Society.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Kouichi Soga
    • 1
    Email author
  • Kazuyuki Wakabayashi
    • 1
  • Seiichiro Kamisaka
    • 2
  • Takayuki Hoson
    • 1
  1. 1.Department of Biology and Geosciences, Graduate School of ScienceOsaka City UniversitySumiyoshi-ku, OsakaJapan
  2. 2.Department of Biology, Faculty of ScienceToyama UniversityGofuku, ToyamaJapan

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