Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana
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Pectin methylesterases (PME, EC. 22.214.171.124) are enzymes that demethylesterify plant cell wall pectins in muro. In Arabidopsis thaliana, putative PME proteins are thought to be encoded by a 66-member gene family. This study used real-time RT-PCR to gain an overview of the expression of the entire family at eight silique developmental stages, in flower buds and in vegetative tissue in the Arabidopsis. Only 15% of the PMEs were not expressed at any of the developmental stages studied. Among expressed PMEs, expression data could be clustered into five distinct groups: 19 PMEs highly or uniquely expressed in floral buds, 4 PMEs uniquely expressed at mid-silique developmental stages, 16 PMEs highly or uniquely expressed in silique at late developmental stages, 16 PMEs mostly ubiquitously expressed, and 1 PME with a specific expression pattern, i.e. not expressed during early silique development. Comparison of expression and phylogenetic profiles showed that, within phylogenetic group 2, all but one PME belong to the floral bud expression group. Similar results were shown for a subset of one of the phylogenetic group, which differed from others by containing most of the PMEs that do not possess any PRO part next to their catalytic part. Expression data were confirmed by two promoter:GUS transgenic plant analysis revealing a PME expressed in pollen and one in young seeds. Our results highlight the high diversity of PME expression profiles. They are discussed with regard to the role of PMEs in fruit development and cell growth.
KeywordsArabidopsis Cell wall Pectin methylesterase Silique Real-time RT-PCR
Reverse transcription polymerase chain reaction
Days after flowering
We thank Sylvain Jeandroz, (University of Nancy I, France) for help with the phylogenetic analysis, the CRRBM (Centre de Ressources Régionales en Biologie Moléculaire) for the use of the Roche LightCycler and the French Ministry of Research for the funding of Romain Louvet’s PhD. The technical assistance of Françoise Fournet is gratefully acknowledged.
- Alexandre F, Morvan O, Gaffe J, Mareck A, Jauneau A, Dauchel H, Balangé AP, Morvan C (1997) Pectin methylesterase pattern in flax seedlings during their development. Plant Physiol Biochem 35:427–436Google Scholar
- Barnavon L, Doco T, Terrier N., Ageorges A, Romieu C, Pellerin P (2001) Involvement of pectin methyl-esterase during the ripening of grape berries: partial cDNA isolation, transcript expression and changes in the degree of methyl-esterification of cell wall pectins. Phytochemistry 58:693–701PubMedCrossRefGoogle Scholar
- Manfield IW, Orfila C, McCartney L, Harholt J, Bernal AJ, Vibe Scheller H, Gilmartin PM, Mikkelsen JD, Knox JP, Willats WGT (2004) Novel cell wall architecture of isobaxen-habituated Arabidopsis suspension cultured cells: global transcript profiling and cellular analysis. Plant J 40:260–275PubMedCrossRefGoogle Scholar
- Wakabayashi K, Hoson T, Huber DJ (2003) Methyl de-esterification as a major factor regulating the extent of pectin depolymerization during fruit ripening: a comparison of the action of avocado (Persea americana) and tomato (Lycopersicon esculentum) polygalacturonases. J Plant Physiol 160:667–673PubMedCrossRefGoogle Scholar
- Willats WG, Orfila C, Limberg G, Buchholt HC, van Alebeek GJ, Voragen AG, Marcus SE, Christensen TM, Mikkelsen JD, Murray BS, Knox JP (2001) Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl esterase action, matrix properties, and cell adhesion. J Biol Chem 276:19404–19413PubMedCrossRefGoogle Scholar