, Volume 224, Issue 3, pp 485–495 | Cite as

Taproot promoters cause tissue specific gene expression within the storage root of sugar beet

  • Heiko Oltmanns
  • Dorothee U. Kloos
  • Waltraud Brieß
  • Maike Pflugmacher
  • Dietmar J. Stahl
  • Reinhard Hehl
Original Article


The storage root (taproot) of sugar beet (Beta vulgaris L.) originates from hypocotyl and primary root and contains many different tissues such as central xylem, primary and secondary cambium, secondary xylem and phloem, and parenchyma. It was the aim of this work to characterize the promoters of three taproot-expressed genes with respect to their tissue specificity. To investigate this, promoters for the genes Tlp, His1-r, and Mll were cloned from sugar beet, linked to reporter genes and transformed into sugar beet and tobacco. Reporter gene expression analysis in transgenic sugar beet plants revealed that all three promoters are active in the storage root. Expression in storage root tissues is either restricted to the vascular zone (Tlp, His1-r) or is observed in the whole organ (Mll). The Mll gene is highly organ specific throughout different developmental stages of the sugar beet. In tobacco, the Tlp and Mll promoters drive reporter gene expression preferentially in hypocotyl and roots. The properties of the Mll promoter may be advantageous for the modification of sucrose metabolism in storage roots.


Beta β-Glucuronidase Luciferase Storage roots Tissue specificity Transgenic plants 



Linker histone variant






Major latex-like gene


Polymerase chain reaction


Thaumatin-like protein


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Heiko Oltmanns
    • 2
  • Dorothee U. Kloos
    • 3
  • Waltraud Brieß
    • 1
  • Maike Pflugmacher
    • 1
  • Dietmar J. Stahl
    • 1
  • Reinhard Hehl
    • 4
  1. 1.PLANTA Angewandte Pflanzengenetik und Biotechnologie GmbHEinbeck Germany
  2. 2.Department of Biological SciencesPurdue UniversityINUSA
  3. 3.Definiens AGMünchenGermany
  4. 4.Institut für GenetikTechnische Universität BraunschweigBraunschweigGermany

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