Planta

, Volume 224, Issue 2, pp 380–393 | Cite as

Influence of the photoperiod on redox regulation and stress responses in Arabidopsis thaliana L. (Heynh.) plants under long- and short-day conditions

  • Beril Becker
  • Simone Holtgrefe
  • Sabrina Jung
  • Christina Wunrau
  • Andrea Kandlbinder
  • Margarete Baier
  • Karl-Josef Dietz
  • Jan E. Backhausen
  • Renate Scheibe
Original Article

Abstract

Arabidopsis thaliana L. (Heynh.) plants were grown in low light (150 μmol photons m−2 s−1 and 20°C) either in short days (7.5 h photoperiod) or long days (16 h photoperiod), and then transferred into high light and low temperature (350–800 μmol photons m−2 s−1 at 12°C). Plants grown in short days responded with a rapid increase in NADP-malate dehydrogenase (EC 1.1.1.82) activation state. However, persisting overreduction revealed a new level of regulation of the malate valve. Activity measurements and Northern-blot analyses indicated that NADP-malate dehydrogenase transcript and protein levels increased within a few hours. Using macroarrays, additional changes in gene expression were identified. Transcript levels for several enzymes of glutathione metabolism and of some photosynthetic genes increased. The cellular glutathione level increased, but its redox state remained unchanged. A different situation was observed in plants grown in long-day conditions. Neither NADP-malate dehydrogenase nor glutathione content changed, but the expression of several antioxidative enzymes increased strongly. We conclude that the endogenous systems that measure day length interact with redox regulation, and override the interpretation of the signals, i.e. they redirect redox-mediated acclimation signals to allow for more efficient light usage and redox poising in short days to systems for the prevention of oxidative damages when grown under long-day conditions.

Keywords

Glutathione Light acclimation Malate valve Oxidative stress Photoperiod Redox regulation 

Abbreviations

Apx

Ascorbate peroxidase

Cat

Catalase

DTT

Dithiothreitol

GR

Glutathione reductase

GS

Glutathione synthase

LD

Long day

LHC

Light-harvesting complex

MDHAR

Monodehydroascorbate reductase

MDH

Malate dehydrogenase

NTR

NADP-thioredoxin reductase

SD

Short day

SOD

Superoxide dismutase

Trx

Thioredoxin

Notes

Acknowledgements

The authors thank H. Rennenberg and M. Eiblmeyer (Universitaet Freiburg, Germany) for their help with the method of glutathione determination. Further thanks are due to S. Klocke for her help with performing the experiments, and R. Brockmann for the frustrating job of measuring the NADP-MDH activities in Arabidopsis plants grown in mixed SD and LD conditions. We finally thank H. Wolf-Wibbelmann and S. Steinbach for excellently growing the plant material. This work was financially supported by a grant from the Deutsche Forschungsgemeinschaft (FOR 387, TP1 and TP3).

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Beril Becker
    • 1
  • Simone Holtgrefe
    • 1
  • Sabrina Jung
    • 1
  • Christina Wunrau
    • 1
  • Andrea Kandlbinder
    • 2
  • Margarete Baier
    • 2
  • Karl-Josef Dietz
    • 2
  • Jan E. Backhausen
    • 1
  • Renate Scheibe
    • 1
  1. 1.Pflanzenphysiologie, Fachbereich Biologie/ChemieUniversität OsnabrückOsnabrückGermany
  2. 2.Lehrstuhl für Biochemie und Physiologie der Pflanzen, Fakultät für BiologieUniversität BielefeldBielefeldGermany

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