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Planta

, Volume 221, Issue 5, pp 739–746 | Cite as

The genome sequence of black cottonwood (Populus trichocarpa) reveals 18 conserved cellulose synthase (CesA) genes

  • Soraya Djerbi
  • Mats Lindskog
  • Lars Arvestad
  • Fredrik Sterky
  • Tuula T. Teeri
Rapid Communication

Abstract

The genome sequence of Populus trichocarpa was screened for genes encoding cellulose synthases by using full-length cDNA sequences and ESTs previously identified in the tissue specific cDNA libraries of other poplars. The data obtained revealed 18 distinct CesA gene sequences in P. trichocarpa. The identified genes were grouped in seven gene pairs, one group of three sequences and one single gene. Evidence from gene expression studies of hybrid aspen suggests that both copies of at least one pair, CesA3-1 and CesA3-2, are actively transcribed. No sequences corresponding to the gene pair, CesA6-1 and CesA6-2, were found in Arabidopsis or hybrid aspen, while one homologous gene has been identified in the rice genome and an active transcript in Populus tremuloides. A phylogenetic analysis suggests that the CesA genes previously associated with secondary cell wall synthesis originate from a single ancestor gene and group in three distinct subgroups. The newly identified copies of CesA genes in P. trichocarpa give rise to a number of new questions concerning the mechanism of cellulose synthesis in trees.

Keywords

Populus Paleopolyploids Cellulose synthase Gene duplication Paralogues 

Abbreviations

CesA

Cellulose synthase

CDS

Coding sequence

EST

Expressed sequence tag

UTR

Untranslated region

Notes

Acknowledgements

We thank Per Unneberg, Peter Savolainen and Peter Nilsson for fruitful discussions and critical reading of the manuscript. The EU project QLK5-CT2001-00443 and the Knut and Alice Wallenberg foundation are gratefully acknowledged for financial support.

Supplementary material

425_2005_1498_ESM_supp.pdf (316 kb)
PDF (761 KB)

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Soraya Djerbi
    • 1
  • Mats Lindskog
    • 1
  • Lars Arvestad
    • 2
  • Fredrik Sterky
    • 1
  • Tuula T. Teeri
    • 1
  1. 1.Royal Institute of Technology, Department of BiotechnologyAlbaNova University CenterStockholmSweden
  2. 2.Stockholm Bioinformatics Center and Royal Institute of Technology, Department of Numerical Analysis and Computer ScienceAlbaNova University CenterStockholmSweden

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