, 224:300 | Cite as

Rice ascorbate peroxidase gene family encodes functionally diverse isoforms localized in different subcellular compartments

  • Felipe Karam Teixeira
  • Larissa Menezes-Benavente
  • Vinícius Costa Galvão
  • Rogério Margis
  • Márcia Margis-Pinheiro
Original Article


Aerobic organisms evolved a complex antioxidant system, which protect the cells against oxidative damage caused by partially reduced oxygen intermediates, also known as reactive oxygen species. In plants, ascorbate peroxidases (EC, catalyze the conversion of H2O2 to H2O, using ascorbate as the specific electron donor in this enzymatic reaction. Previously, eight APx genes were identified in the rice (Oryza sativa L.) genome through in silico analysis: two cytosolic isoforms, two putative peroxisomal isoforms, and four putative chloroplastic ones. Using gene-specific probes, we confirmed the presence of the eight APx genes in the rice genome by Southern blot hybridization. Transcript accumulation analysis showed specific expression patterns for each member of the APx family according to developmental stage and in response to salt stress, revealing the complexity of the antioxidant system in plants. Finally, the subcellular localization of rice APx isoforms was determined using GFP-fusion proteins in BY-2 tobacco cells. In agreement with the initial prediction, OSAPX3 was localized in the peroxisomes. On the other hand, the OSAPX6-GFP fusion protein was found in mitochondria of the BY-2 cells, in contrast to the chloroplastic location predicted by sequence analysis. Our findings reveal the functional diversity of the rice APx genes and suggest complementation and coordination of the antioxidant defenses in different cellular compartments during development and abiotic stress.


Antioxidant metabolism Ascorbate peroxidase BY-2 tobacco cells Green fluorescent protein Oryza 



Ascorbate peroxidase


Cauliflower mosaic virus




Dehydroascorbate reductase


Differential interference contrast


Expressed sequence tag


Green fluorescent protein


Glutathione peroxidase


Glutathione reductase


Knock-out mutants


Monodehydroascorbate reductase


Programmed cell death


Peroxisomal endoplasmatic reticulum




Reactive oxygen species


Superoxide dismutase


Splicing regulatory cis-element


Tetramethylrhodamine ethyl ester


3´untranslated region


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Felipe Karam Teixeira
    • 1
  • Larissa Menezes-Benavente
    • 1
    • 4
  • Vinícius Costa Galvão
    • 1
  • Rogério Margis
    • 2
  • Márcia Margis-Pinheiro
    • 1
    • 3
  1. 1.Laboratório de Genética Molecular Vegetal, Departamento de GenéticaUniversidade Federal do Rio de JaneiroRio de JaneiroBrasil
  2. 2.Departamento de BioquímicaUniversidade Federal do Rio Grande do SulRio Grande do SulBrasil
  3. 3.Laboratório de Genética Vegetal, Departamento de GenéticaUniversidade Federal do Rio Grande do Sul. Av. Bento GonçalvesPorto AlegreBrasil
  4. 4.Department of GeneticsNorth Carolina State UniversityRaleighUSA

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