, Volume 223, Issue 2, pp 369–373 | Cite as

Production of rosmarinic acid and a new rosmarinic acid 3′-O-β-D-glucoside in suspension cultures of the hornwort Anthoceros agrestis Paton

  • Katharina Vogelsang
  • Bernd Schneider
  • Maike Petersen
Original Article


Cell suspension cultures of the hornwort Anthoceros agrestis Paton (Anthocerotaceae) were cultivated and characterized in CB-media containing 2 and 4% sucrose. The suspension cells accumulated rosmarinic acid up to 5.1% of the cell dry weight as well as caffeoyl-4′-hydroxyphenyllactate. Moreover, a more hydrophilic compound was detected which was isolated and identified as rosmarinic acid 3′-O-β-D-glucoside, a new rosmarinic acid derivative. This new rosmarinic acid derivative was found up to 1.0% of the cell dry weight in suspension cells of A. agrestis.


Anthoceros agrestis Paton (Anthocerotaceae) Hornworts Rosmarinic acid Rosmarinic acid 3′-O-β-glucoside Suspension cultures 



We thank Dr. Aleš Svatoš (Jena, Germany) for mass spectrometric analysis.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Katharina Vogelsang
    • 1
  • Bernd Schneider
    • 2
  • Maike Petersen
    • 1
  1. 1.Institut für Pharmazeutische BiologiePhilipps-Universität MarburgMarburgGermany
  2. 2.Max-Planck-Institut für Chemische ÖkologieBeutenberg CampusJenaGermany

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