Planta

, Volume 223, Issue 2, pp 223–236

Cell cycle-dependent changes in Golgi stacks, vacuoles, clathrin-coated vesicles and multivesicular bodies in meristematic cells of Arabidopsis thaliana: A quantitative and spatial analysis

Original Article

Abstract

Cytokinesis in plants involves both the formation of a new wall and the partitioning of organelles between the daughter cells. To characterize the cellular changes that accompany the latter process, we have quantitatively analyzed the cell cycle-dependent changes in cell architecture of shoot apical meristem cells of Arabidopsis thaliana. For this analysis, the cells were preserved by high-pressure freezing and freeze-substitution techniques, and their Golgi stacks, multivesicular bodies, vacuoles and clathrin-coated vesicles (CCVs) characterized by means of serial thin section reconstructions, stereology and electron tomography techniques. Interphase cells possess ∼35 Golgi stacks, and this number doubles during G2 immediately prior to mitosis. At the onset of cytokinesis, the stacks concentrate around the periphery of the growing cell plate, but do not orient towards the cell plate. Interphase cells contain ∼18 multivesicular bodies, most of which are located close to a Golgi stack. During late cytokinesis, the appearance of a second group of cell plate-associated multivesicular bodies coincides with the onset of CCV formation at the cell plate. During this period a 4× increase in CCVs is paralleled by a doubling in number and a 4× increase in multivesicular bodies volume. The vacuole system also undergoes major changes in organization, size, and volume, with the most notable change seen during early telophase cytokinesis. In particular, the vacuoles form sausage-like tubular compartments with a 50% reduced surface area and an 80% reduced volume compared to prometaphase cells. We postulate that this transient reduction in vacuole volume during early telophase provides a means for increasing the volume of the cytosol to accommodate the forming phragmoplast microtubule array and associated cell plate-forming structures.

Keywords

Clathrin-coated vesicles Electron tomography Golgi Multivesicular bodies Stereology (shoot meristem cells) Arabidopsis 

Abbreviations

CCV

Clathrin-coated vesicle

dn

Mean nuclear diameter

Dn

Corrected mean nuclear diameter

EM

Electron microscopy

ER

Endoplasmic reticulum

MVB

Multivesicular body

Na

Plane numerical density

Nv

Numerical density

Sv

Surface density

TGN

Trans-Golgi network

Vv

Volume density

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • José M. Seguí-Simarro
    • 1
    • 2
  • L. Andrew Staehelin
    • 1
  1. 1.Department of Molecular, Cellular and Developmental BiologyUniversity of ColoradoBoulderUSA
  2. 2.Centro de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV)Universidad Politécnica de ValenciaValenciaSpain

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