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Planta

, Volume 222, Issue 4, pp 613–622 | Cite as

QUASIMODO1 is expressed in vascular tissue of Arabidopsis thaliana inflorescence stems, and affects homogalacturonan and xylan biosynthesis

  • Caroline Orfila
  • Susanne Oxenbøll Sørensen
  • Jesper Harholt
  • Naomi Geshi
  • Hazel Crombie
  • Hoai-Nam Truong
  • J. S. Grant Reid
  • J. Paul Knox
  • Henrik Vibe Scheller
Original Article

Abstract

An insertion in the promoter of the Arabidopsis thaliana QUA1 gene (qua1-1 allele) leads to a dwarf plant phenotype and a reduction in cell adhesion, particularly between epidermal cells in seedlings and young leaves. This coincides with a reduction in the level of homogalacturonan epitopes and the amount of GalA in isolated cell walls (Bouton et al., Plant Cell 14: 2577 2002). The present study was undertaken in order to investigate further the link between QUA1 and cell wall biosynthesis. We have used rapidly elongating inflorescence stems to compare cell wall biosynthesis in wild type and qua1-1 mutant tissue. Relative to the wild type, homogalacturonan α-1-4-D-galacturonosyltransferase activity was consistently reduced in qua1-1 stems (by about 23% in microsomal and 33% in detergent-solubilized membrane preparations). Activities of β-1-4-D-xylan synthase, β-1-4-D-galactan synthase and β-glucan synthase II activities were also measured in microsomal membranes. Of these, only β-1-4-D-xylan synthase was affected, and was reduced by about 40% in qua1-1 stems relative to wild type. The mutant phenotype was apparent in inflorescence stems, and was investigated in detail using microscopy and cell wall composition analyses. Using in situ PCR techniques, QUA1 mRNA was localized to discrete cells of the vascular tissue and subepidermal layers. In mutant stems, the organization of these tissues was disrupted and there was a modest reduction in homogalacturonan (JIM5) epitopes. This study demonstrates a specific role for QUA1 in the development of vascular tissue in rapidly elongating inflorescence stems and supports a role of QUA1 in pectin and hemicellulose cell wall synthesis through affects on α-1,4-D-galacturonosyltransferase and β-1,4-D-xylan synthase activities.

Keywords

Cell wall Galacturonic acid Galacturonosyltransferase Glycosyltransferase Pectin Xylosyltransferase 

Abbreviations

GalA

Galacturonic acid

HG

Homogalacturonan

RG

Rhamnogalacturonan

Notes

Acknowledgements

The authors wish to acknowledge Charlotte Sørensen for technical assistance and Dr. Tove Christensen and Dr. Troels Gravesen (Danisco Ingredients, Copenhagen, Denmark) for the generous gift of cell wall degrading enzymes. Dr. Herman Höfte (INRA, Versailles, France) and Jacob Krüger Jensen (The Royal Veterinary and Agricultural University, Copenhagen, Denmark) are thanked for critical reading of the manuscript and discussions. This work was supported in part by the European Union Fifth Framework Programme (QLK3-CT-1999-00089, Europectin) and by the Danish National Research Foundation.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Caroline Orfila
    • 1
    • 6
  • Susanne Oxenbøll Sørensen
    • 1
  • Jesper Harholt
    • 1
  • Naomi Geshi
    • 2
  • Hazel Crombie
    • 3
  • Hoai-Nam Truong
    • 4
  • J. S. Grant Reid
    • 3
  • J. Paul Knox
    • 5
  • Henrik Vibe Scheller
    • 1
  1. 1.Plant Biochemistry Laboratory, Department of Plant BiologyThe Royal Veterinary and Agricultural UniversityFrederiksberg CDenmark
  2. 2.Biotechnology GroupDanish Institute of Agricultural SciencesFrederiksberg CDenmark
  3. 3.School of Biological and Environmental SciencesUniversity of StirlingStirlingUK
  4. 4.Unité de Nutrition Azotée des PlantesInstitut National de la Recherche Agronomique Centre de Versailles-GrillonVersailles CedexFrance
  5. 5.Centre for Plant Sciences, Faculty of Biological SciencesUniversity of LeedsLeedsUK
  6. 6.School of Applied SciencesNorthumbria UniversityNewcastle Upon TyneUK

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