The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14λ and the PP2C phosphatase KAPP
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Leucine-rich repeat (LRR)-containing transmembrane receptor-like kinases (RLKs) are important components of plant signal transduction. The Arabidopsis thaliana somatic embryogenesis receptor-like kinase 1 (AtSERK1) is an LRR-RLK proposed to participate in a signal transduction cascade involved in embryo development. By yeast two-hybrid screening we identified AtCDC48, a homologue of the mammalian AAA-ATPase p97 and GF14λ, a member of the Arabidopsis family of 14-3-3 proteins as AtSERK1 interactors. In vitro, the AtSERK1 kinase domain is able to transphosphorylate and bind both AtCDC48 and GF14λ. In yeast, AtCDC48 interacts with GF14λ and with the PP2C phosphatase KAPP. In plant protoplasts AtSERK1 interacts with GF14λ.
Keywords14-3-3 Protein Arabidopsis Cell division cycle protein 48 Kinase associated protein phosphatase (KAPP) Signal transduction Somatic embryogenesis receptor-like kinase 1
Maltose binding protein
Förster resonance energy transfer
Fluorescence spectral imaging microscopy
Cyan fluorescent protein
Yellow fluorescent protein
We are grateful to the following colleagues from Wageningen University, Laboratories of Biochemistry and Molecular Biology: Casper Vroemen and Vered Raz for the many useful discussions and critical comments on the manuscript, Ingrid Vleghels for her help with the yeast two-hybrid system, Jeroen Pouwels for his help with isolation and transformation of the cowpea protoplasts, Boudewijn van Veen, Jacques Vervoort and Olga Kulikova for their help with the figures in the manuscript and Gerard van der Krogt for providing us with the YFP/CFP cloning vectors. This work was supported by grant 805.18.302 from the Netherlands Organisation for Scientific Research NWO (I. M. R), grant QLG2-2000-00603 from the EU Quality of Life and Management of Living Resources program (E. R).
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