Planta

, Volume 220, Issue 1, pp 38–46 | Cite as

Nuclear fusions contribute to polyploidization of the gigantic nuclei in the chalazal endosperm of Arabidopsis

Original Article

Abstract

Somatic polyploidization is recognized as a means to increase gene expression levels in highly active metabolic cells. The most common mechanisms are endoreplication, endomitosis and cell fusion. In animals and plants the nuclei of multinucleate cells are usually prevented from fusing. Here, we report that the nuclei from the syncytial cyst of the chalazal endosperm of Arabidopsis thaliana (L.) Heynh. are polyploid with some intermediate ploidy levels that cannot be attributed to endoreplication, suggesting nuclear fusion. Analysis of isolated nuclei, together with fluorescent in situ hybridization (FISH), revealed that nuclei from the chalazal endosperm are two or three times bigger than the nuclei from the peripheral endosperm and have a corresponding increase in ploidy. Together with the consistent observation of adjoined nuclei, we propose that nuclear fusion contributes, at least in part, to the process of polyploidization in the chalazal endosperm. Confocal analysis of intact seeds further suggested that free nuclei from the peripheral endosperm get incorporated into the chalazal cyst and likely participate in nuclear fusions.

Keywords

Arabidopsis Chalazal endosperm FISH Nuclear fusion Polyploidization 

Abbreviations

BAC

Bacterial artificial chromosome

CZE

Chalazal endosperm

DAPI

4,6-Diamino-2-phenylindole

FISH

Fluorescent in situ hybridization

NOR

Nucleolar organizing region

NCD

Nuclear cytoplasmic domain

PEN

Peripheral endosperm

Notes

Acknowledgements

We gratefully acknowledge Philippa Barrell for the protocol of improved Feulgen staining and for comments on the manuscript, and two anonymous reviewers for helpful suggestions. C.B. was supported by a fellowship from the Roche Research Foundation; the project was also supported by the University of Zürich and a Searle Scholarship to U.G.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Institute of Plant Biology & Zürich-Basel Plant Science CenterUniversity of ZürichZürichSwitzerland
  2. 2.Swammerdam Institute for Life SciencesUniversity of AmsterdamSM AmsterdamThe Netherlands

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