Planta

, Volume 219, Issue 6, pp 1036–1041 | Cite as

A monoclonal antibody to feruloylated-(1→4)-β-d-galactan

  • Mads H. Clausen
  • Marie-Christine Ralet
  • William G. T. Willats
  • Lesley McCartney
  • Susan E. Marcus
  • Jean-François Thibault
  • J. Paul Knox
Original Article

Abstract

We report the isolation and characterization of a monoclonal antibody, designated LM9, against feruloylated-(1→4)-β-d-galactan. This epitope is a structural feature of cell wall pectic polysaccharides of plants belonging to the family Amaranthaceae (including the Chenopodiaceae). Immuno-assays and immunofluorescence microscopy indicated that LM9 binding is specific to samples and cell walls obtained from species belonging to this family. In a series of competitive-inhibition enzyme-linked immunosorbent assays with potential oligosaccharide haptens, the most effective inhibitor was O-[6-O-(trans-feruloyl)-β-d-galactopyranosyl]-(1→4)-d-galactopyranose (Gal2F). LM9 is therefore a useful antibody probe for the analysis of phenolic substitution of cell wall pectic polymers and of cell wall structure in the Amaranthaceae including sugar beet (Beta vulgaris L.) and spinach (Spinacia oleracea L.).

Keywords

Amaranthaceae (Chenopodiaceae) Cell wall Feruloylated galactan Pectin (monoclonal antibody) 

Abbreviations

DA

Degree of acetylation

DM

Degree of methyl esterification

ELISA

Enzyme-linked immunosorbent assay

IDA

Immunodot assay

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Mads H. Clausen
    • 1
    • 2
  • Marie-Christine Ralet
    • 3
  • William G. T. Willats
    • 1
    • 4
  • Lesley McCartney
    • 1
  • Susan E. Marcus
    • 1
  • Jean-François Thibault
    • 3
  • J. Paul Knox
    • 1
  1. 1.Centre for Plant SciencesUniversity of LeedsLeedsUK
  2. 2.Department of ChemistryTechnical University of DenmarkKgs. LyngbyDenmark
  3. 3.Unité de Recherche sur les Polysaccharides, leurs Organisations et InteractionsInstitut National de la Recherche AgronomiqueNantes Cedex 03France
  4. 4.Institute of Molecular BiologyUniversity of CopenhagenCopenhagenDenmark

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