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Planta

, Volume 219, Issue 3, pp 428–439 | Cite as

Photoinhibition and recovery in a herbicide-resistant mutant from Glycine max (L.) Merr. cell cultures deficient in fatty acid unsaturation

  • Miguel AlfonsoEmail author
  • Raquel Collados
  • Inmaculada Yruela
  • Rafael Picorel
Original Article

Abstract

Photoinhibition and recovery were studied in two photosynthetic cell suspensions from soybean (Glycine max L. Merr): the wild type (WT) and the herbicide-resistant D1 mutant STR7. This mutant also showed an increase in saturated fatty acids from thylakoid lipids. STR7 was more sensitive to photoinhibition under culture conditions. In vivo photoinhibition experiments in the presence of chloramphenicol, in vitro studies in isolated thylakoid membranes, and immunoblot analysis indicated that the process of light-induced degradation of the D1 protein was not involved in the response of STR7 to light. At growth temperature (24°C), the recovery rate of photoinhibited photosystem II (PSII) was slower in STR7 relative to WT. Photoinhibition and recovery were differentially affected by temperature in both cell lines. The rates of photoinhibition were faster in STR7 at any temperature below 27°C. The rates of PSII recovery from STR7 were more severely affected than those of WT at temperatures lower than 24°C. The photoinhibition and recovery rates of WT at 17°C mimicked those of STR7 at 24°C. In organelle translation studies indicated that synthesis and elongation of D1 were substantially similar in both cell lines. However, sucrose gradient fractionation of chloroplast membranes demonstrated that D1 and also other PSII proteins such as D2, OEE33, and LCHII had a reduced capability to incorporate into PSII to yield a mature assembled complex in STR7. This effect may become the rate-limiting step during the recovery of photoinhibited PSII and may explain the increased sensitivity to high light found in STR7. Our data may hint at a possible role of fatty acids from membrane lipids in the assembly and dynamics of PSII.

Keywords

Cell culture Fatty acid unsaturation Glycine Herbicide resistance Light stress Photosystem II 

Abbreviations

DCBQ

2,6-Dichloro p-benzoquinone

DM

Dodecyl-β-d-maltoside

DTT

Dithiothreitol

HL

High light

LHCII

Light-harvesting complex II

LL

Low light

OEE

Oxygen-evolving extrinsic proteins

PAGE

Polyacrylamide gel electrophoresis

PG

Phosphatidylglycerol

PSII

Photosystem II

QA and QB

Secondary quinone electron acceptors from PSII

RC

Reaction center

SDS

Sodium dodecyl sulfate

WT

Wild type

Notes

Acknowledgements

This work was supported by the MCYT (Grants PB98-1632 and BMC2002-00031). M.A. is the recipient of a contract from MCYT (Programa Ramón y Cajal) and R.C. is the recipient of a fellowship from Gobierno de Aragón (CONSI+D-DGA). Technical assistance by María V. Ramiro is also gratefully acknowledged.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Miguel Alfonso
    • 1
    Email author
  • Raquel Collados
    • 1
  • Inmaculada Yruela
    • 1
  • Rafael Picorel
    • 1
  1. 1.Department of Plant NutritionEstación Experimental de Aula Dei (CSIC)ZaragozaSpain

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