Planta

, Volume 218, Issue 4, pp 673–681

A xylogalacturonan epitope is specifically associated with plant cell detachment

  • William G. T. Willats
  • Lesley McCartney
  • Clare G. Steele-King
  • Susan E. Marcus
  • Andrew Mort
  • Miranda Huisman
  • Gert-Jan van Alebeek
  • Henk A. Schols
  • Alphons G. J. Voragen
  • Angélique Le Goff
  • Estelle Bonnin
  • Jean-François Thibault
  • J. Paul Knox
Original Article

DOI: 10.1007/s00425-003-1147-8

Cite this article as:
Willats, W.G.T., McCartney, L., Steele-King, C.G. et al. Planta (2004) 218: 673. doi:10.1007/s00425-003-1147-8

Abstract

A monoclonal antibody (LM8) was generated with specificity for xyloglacturonan (XGA) isolated from pea (Pisum sativum L.) testae. Characterization of the LM8 epitope indicates that it is a region of XGA that is highly substituted with xylose. Immunocytochemical analysis indicates that this epitope is restricted to loosely attached inner parenchyma cells at the inner face of the pea testa and does not occur in other cells of the testa. Elsewhere in the pea seedling, the LM8 epitope was found only in association with root cap cell development at the root apex. Furthermore, the LM8 epitope is specifically associated with root cap cells in a range of angiosperm species. In embryogenic carrot suspension cell cultures the epitope is abundant at the surface of cell walls of loosely attached cells in both induced and non-induced cultures. The LM8 epitope is the first cell wall epitope to be identified that is specifically associated with a plant cell separation process that results in complete cell detachment.

Keywords

Cell wall Pectin (monoclonal antibody) Plant cell adhesion Plant cell detachment Xylogalacturonan 

Abbreviations

DAA

Days after anthesis

2,4-D

2,4-Dichlorophenoxyacetic acid

ELISA

Enzyme-linked immunosorbent assay

GalA

Galacturonic acid

HGA

Homogalacturonan

HPAEC

High-performance anion-exchange chromatography

HPSEC

High-performance size-exclusion chromatography

RG-I

Rhamnogalacturonan-I

RG-II

Rhamnogalacturonan-II

XGA

Xylogalacturonan

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • William G. T. Willats
    • 1
    • 5
  • Lesley McCartney
    • 1
  • Clare G. Steele-King
    • 1
  • Susan E. Marcus
    • 1
  • Andrew Mort
    • 2
  • Miranda Huisman
    • 3
  • Gert-Jan van Alebeek
    • 3
  • Henk A. Schols
    • 3
  • Alphons G. J. Voragen
    • 3
  • Angélique Le Goff
    • 4
  • Estelle Bonnin
    • 4
  • Jean-François Thibault
    • 4
  • J. Paul Knox
    • 1
  1. 1.Centre for Plant SciencesUniversity of LeedsLeedsUK
  2. 2.Department of Biochemistry and Molecular BiologyOklahoma State UniversityStillwaterUSA
  3. 3.Department of Agrotechnology and Food SciencesWageningen UniversityEV WageningenThe Netherlands
  4. 4.Unité de Recherche sur les Polysaccharides, leurs Organisations et InteractionsINRANantes Cedex 03France
  5. 5.Department of Plant Physiology, Institute of Molecular BiologyUniversity of CopenhagenCopenhagen KDenmark

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