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Planta

, Volume 218, Issue 1, pp 87–97 | Cite as

Slippery surfaces of carnivorous plants: composition of epicuticular wax crystals in Nepenthes alata Blanco pitchers

  • Michael Riedel
  • Anna Eichner
  • Reinhard Jetter
Original Article

Abstract

Plants in the genus Nepenthes obtain a substantial nutrient supply by trapping insects in highly modified leaves. A broad zone of the inner surface of these pitchers is densely covered with wax crystals on which most insects lose their footing. This slippery wax surface, capturing prey and preventing its escape from the trap, plays a pivotal role in the carnivorous syndrome. To understand the mechanism of slipperiness, the present investigation aimed at an ultrastructural and physico-chemical characterization of the wax crystals in pitchers of N. alata Blanco. Scanning electron microscopy revealed that entire platelets protruded perpendicularly from the surface. Methods were developed that allowed the mechanical removal of wax crystals from the pitcher surface. It could be shown that the sampling was selective for the epicuticular wax, relevant for plant–insect interactions. The crystals consisted of a mixture of aliphatic compounds dominated by very-long-chain aldehydes. Triacontanal, at 43% the most abundant constituent, was largely responsible for crystal formation. Solubility data indicate that the Nepenthes crystals contained polymeric forms of this aldehyde. The resulting mechanical properties of the polymer crystals and the mechanism of slipperiness are discussed.

Keywords

Carnivorous plants Epicuticular wax crystals Insect attachment Mechanical wax removal Nepenthes Plant–insect interaction 

Abbreviation

SEM

scanning electron microscopy

Notes

Acknowledgements

The authors are indebted to Prof. Markus Riederer (University of Würzburg, Department of Botany II) and Dr. Walter Federle (University of Würzburg, Department of Zoology II) for fruitful discussions. Technical assistance by Stefanie Schäffer, Bianka Pink (both Department of Botany II) and the staff of the Botanical Garden of the University of Würzburg is gratefully acknowledged. Cornelia P. Vermeer and Peter Schmid (both Department of Botany II) provided data and standards. This work has been supported by the SFB 567 'Mechanisms of interspecific interactions of organisms'.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Michael Riedel
    • 1
  • Anna Eichner
    • 1
  • Reinhard Jetter
    • 1
    • 2
  1. 1.Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik IIUniversität WürzburgWürzburgGermany
  2. 2.Departments of Botany and ChemistryUBCVancouverCanada

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