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Planta

, Volume 216, Issue 1, pp 187–192 | Cite as

The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway

  • Bernadette von Malek
  • Eric van der Graaff
  • Kay Schneitz
  • Beat Keller
Original Article

Abstract.

The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.

ALLENE OXIDE SYNTHASE gene Arabidopsis mutant Candidate gene Jasmonate Male sterility 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Bernadette von Malek
    • 1
  • Eric van der Graaff
    • 1
  • Kay Schneitz
    • 1
  • Beat Keller
    • 1
  1. 1.Institute of Plant Biology, University of Zurich, Zollikerstr. 107, 8008 Zurich, Switzerland

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