Planta

, Volume 215, Issue 3, pp 518–522

A WEE1 homologue from Arabidopsis thaliana

  • David A. Sorrell
  • Angela Marchbank
  • Kathryn McMahon
  • Richard J. Dickinson
  • Hilary J. Rogers
  • Dennis Francis
Rapid Communication

DOI: 10.1007/s00425-002-0815-4

Cite this article as:
Sorrell, D.A., Marchbank, A., McMahon, K. et al. Planta (2002) 215: 518. doi:10.1007/s00425-002-0815-4

Abstract.

Little is known about the genes that regulate cyclinB–Cdc2 complexes at the G2/M transition of the plant cell cycle although in yeast and animals cdc25 and wee1 are central regulators of cdc2. Here we describe the isolation, by reverse transcription polymerase chain reaction (RT-PCR), of a WEE1 cDNA (AtWEE1) in Arabidopsis thaliana (L.) Heynh. Semi-quantitative RT-PCR showed that AtWEE1 expression was confined to actively dividing regions of the plant. The overexpression of AtWEE1 in fission yeast (Schizosaccharomyces pombe) caused cells to arrest, and to grow but not divide, resulting in very elongated cells. Our data provide evidence for a functional WEE1 in A. thaliana.

Arabidopsis (cell cycle) Cell cycle Cyclin-dependent kinase Schizosaccharomyces wee1 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • David A. Sorrell
    • 2
  • Angela Marchbank
    • 1
  • Kathryn McMahon
    • 3
  • Richard J. Dickinson
    • 1
  • Hilary J. Rogers
    • 1
  • Dennis Francis
    • 1
  1. 1.School of Biosciences, Cardiff University, PO Box 915, Cardiff, CF10 3TL, UK
  2. 2.Hannah Research Institute, Ayr, KA6 5HL UK
  3. 3.Department of Plant Sciences, Oxford University, South Parks Road, Oxford, OX1 3RB, UK

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