Planta

, Volume 215, Issue 2, pp 339–344

Antisense and chemical suppression of the nonmevalonate pathway affects ent-kaurene biosynthesis in Arabidopsis

  • Kazunori Okada
  • Hiroshi Kawaide
  • Tomohisa Kuzuyama
  • Haruo Seto
  • Ian S. Curtis
  • Yuji Kamiya
Rapid Communication

DOI: 10.1007/s00425-002-0762-0

Cite this article as:
Okada, K., Kawaide, H., Kuzuyama, T. et al. Planta (2002) 215: 339. doi:10.1007/s00425-002-0762-0

Abstract.

Transgenic plants of Arabidopsis thaliana (L.) Heynh. (ecotype Columbia) expressing the antisense AtMECT gene, encoding 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, were generated to elucidate the physiological role of the nonmevalonate pathway for production of ent-kaurene, the latter being the plastidic precursor of gibberellins. In transformed plants pigmentation and accumulation of ent-kaurene were reduced compared to wild-type plants. Fosmidomycin, an inhibitor of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), caused a similar depletion of these compounds in transgenic plants. These observations suggest that both AtMECT and DXR are important in the synthesis of isopentenyl diphosphate and dimethylallyl diphosphate and that ent-kaurene is mainly produced through the nonmevalonate pathway in the plastid.

Arabidopsis ent-Kaurene Fosmidomycin Gibberellin Isoprenoids Nonmevalonate pathway 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Kazunori Okada
    • 1
  • Hiroshi Kawaide
    • 2
  • Tomohisa Kuzuyama
    • 3
  • Haruo Seto
    • 3
  • Ian S. Curtis
    • 2
  • Yuji Kamiya
    • 2
  1. 1.Department of Biology, Tokyo Gakugei University, Nukuikitamachi 4-1-1, Koganei-shi, Tokyo, 184-8501, Japan
  2. 2.Plant Science Center, RIKEN, Hirosawa 2-1, Wako-shi, Saitama, 351-0198, Japan
  3. 3.Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan

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