Pflügers Archiv

, Volume 438, Issue 5, pp 635–641 | Cite as

Interaction between calcium-activated chloride channels and the cystic fibrosis transmembrane conductance regulator

  • L. Wei
  • A. Vankeerberghen
  • H. Cuppens
  • J. Eggermont
  • J.-J. Cassiman
  • G. Droogmans
  • B. Nilius
Original Article

Abstract.

We investigated interactions between cystic fibrosis conductance regulator (CFTR) and endogenous Ca2+-activated Cl channels (CaCC) in bovine pulmonary artery endothelium (CPAE). CPAE cells, which do not express CFTR, were transiently transfected with wild-type (WT) CFTR and the deletion mutant ΔF508 CFTR. Currents through CaCC were significantly reduced after expression of WT CFTR. This inhibition was increased by stimulation (isobutylmethylxanthine, forskolin) of CFTR in cells expressing WT CFTR. There were no such effects when ΔF508 mutant CFTR, which is retained in the endoplasmic reticulum, was expressed. It is concluded that CFTR and CaCC are functionally coupled probably through a direct channel–channel interaction.

Calcium Channel–channel interaction Chloride channels Cystic fibrosis transmembrane conductance regulator Endothelium Green fluorescent protein 

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Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • L. Wei
    • 1
  • A. Vankeerberghen
    • 2
  • H. Cuppens
    • 2
  • J. Eggermont
    • 1
  • J.-J. Cassiman
    • 2
  • G. Droogmans
    • 1
  • B. Nilius
    • 1
  1. 1.Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, Herestraat 49, B-3000 Leuven, Belgium
  2. 2.Center for Human Genetics, Campus Gasthuisberg, KU Leuven, Herestraat 49, B-3000 Leuven, Belgium

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