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Pflügers Archiv

, Volume 443, Issue 2, pp 323–330 | Cite as

Local photolysis using tapered quartz fibres

  • Christoph Eberius
  • Detlev Schild
Instruments and Techniques

Abstract

We describe a versatile, low-cost photolysis system in which sub-millisecond flashes generated by a xenon flash lamp are conveyed through a silica fibre directly onto a cell. The fibre, which is tapered to a sub-micrometre diameter and sputtered with chromium and aluminium, illuminates an area of about 1 µm2. Site and timing of photolysis can be chosen independently from other experimental parameters. As an application example we show the spatially heterogeneous distribution of Ca2+-dependent Cl channels in olfactory receptor neurons.

Flash photolysis Olfactory receptor neurons Calcium-activated chloride current Xenopus laevis 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Christoph Eberius
    • 1
  • Detlev Schild
    • 1
  1. 1.Physiologisches Institut, Universität Göttingen, Humboldtallee 23, 37073 GöttingenGermany

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