Advertisement

Pflügers Archiv

, Volume 438, Issue 3, pp 419–422 | Cite as

Modulation of HERG potassium channel properties by external pH

  • Jocelyn Bérubé
  • Mohamed Chahine
  • P. Daleau
Short Communication

Abstract 

We expressed the human eag-related gene (HERG), known to encode for the cardiac potassium channel IKr, in Chinese hamster ovary (CHO) cells. This study investigated effects of external pH (pHo) on HERG current properties using the whole-cell patch-clamp technique. We observed that current amplitude was decreased and kinetics of activation and deactivation were faster when pHo was lowered from 7.4 to 6.0, while activation was accelerated and V1/2 negatively shifted when pHo was changed from 7.4 to 8.0. These effects can be explained by surface charge screening, amino acid group titration and/or changes in ionic atmosphere. We conclude that alterations of HERG channel by pHo could have consequences for the onset of arrhythmias during cardiac ischemia.

Key words Cardiac repolarization External pH HERG Potassium channels 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Jocelyn Bérubé
    • 1
  • Mohamed Chahine
    • 1
  • P. Daleau
    • 1
  1. 1.Quebec Heart Institute, Laval Hospital, Research Center, 2725 Chemin Ste-Foy, Ste-Foy, QC, Canada, G1V 4G5 e-mail: Pascal.Daleau@phc.ulaval.ca Fax: +1-418-6564509CA

Personalised recommendations