Pflügers Archiv

, Volume 439, Supplement 1, pp r119–r121

Tissue expression and immunolocalization of a novel human cathepsin P

  • Jože Pungerčar
  • Alenka Viyjak
  • Gabriela Ivanovski
  • Igor Križaj
Regular article

Abstract

The mRNA of a novel human cathepsin P is expressed at high levels in lung, liver and heart. Using antibodies raised against recombinant cathepsin P produced in Escherichia coli, a single protein band of 33 kDa was detected by immunoblotting an extract of human liver. By immunofluorescence, positive signals were observed in hepatocytes and Kupffer cells of liver, and the distal tubule cells of kidney showing mainly perimembranous distribution, indicating a role, as yet unknown, for this novel putative protease that is distinct from other cathepsins of the papain family.

Key words Human Liver Kidney Tissue distribution Immunofluorescence 

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • Jože Pungerčar
    • 1
  • Alenka Viyjak
    • 2
  • Gabriela Ivanovski
    • 1
  • Igor Križaj
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, Jožef Stefan Institute, Jamova 39 and
  2. 2.Institute of Pathology, Medical Faculty, Korytkova ulica 2, SI-1000 Ljubljana, SloveniaSI

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