RIM2α/RBP2/β-subunit co-expression stabilizes slow Cav1.3 channel inactivation to improve auditory perception
- 114 Downloads
Cav1.3 channels are voltage-gated L-type Ca2+ channels that are highly expressed in cochlear inner hair cells (IHCs) . The channels are localized predominantly in the active zones of presynaptic ribbons of IHCs and carry most of the Ca2+ currents (> 90%) required for transforming sound-evoked depolarization into graded Ca2+-dependent glutamate release at the IHC-auditory nerve synapse . Cav1.3 channels activate at relatively negative potentials and exhibit fast activation and very slow inactivation kinetics [5, 7, 12]. In this way, they allow fast and sustained Ca2+ signals in a broad range of voltages, making them suitable to transform sound-evoked IHC receptor potential into glutamate release at the postsynaptic ending of auditory nerves. While fast activation and a negative activation voltage range are typical hallmarks of Cav1.3 channels in a variety of excitable cells [3, 7, 8], their slow inactivation kinetics is unique to IHCs. As other voltage-gated Ca2+ channels, Cav1.3 channel inactivation is both Ca2+ (CDI)- and voltage (VDI)-dependent . CDI is fast and calmodulin (CaM)-dependent. CaM, activated by Ca2+, binds to the N- and C-terminal motifs of the pore-forming α1-subunit and inactivates the open channel. VDI of Cav1.3 channels is the predominant inactivation mechanism and is measured by replacing Ca2+ with Ba2+ to prevent Ca2+-dependent CaM activation . In IHCs, VDI is ultra-slow while CDI is nearly absent since mediated by CaBP2, a CaM-like Ca2+-binding protein abundantly expressed in IHCs that prevents CaM-mediated CDI .
Although it is known that accessory β-subunits and other intracellular proteins can modify Cav1.3 channel inactivation , the underlying molecular components regulating the slow VDI in IHCs are not yet fully identified. For instance, the major β-isoforms expressed in IHCs (β2a and β2e) are known to slow VDI to a greater degree than β3, which has been consistently detected in IHCs . Proteins of the presynaptic active zone, such as bassoon, RIM, and RIM-binding proteins (RBPs)  are also able to slow VDI of Cav1.3 in IHC. These proteins favor Cav1.3 channels positioning close to the readily releasable synaptic vesicles at the IHC ribbon synapse and can therefore tightly regulate Cav1.3 channel inactivation kinetics. This hypothesis is supported by previous observation by Joerg Striessnig laboratory  that RIM2α binding to the β3-subunit stabilizes slow Cav1.3 VDI but not to the extent as observed in IHCs.
- 10.Ortner NJ, Pinggera A, Hofer NT, Brandt N, Raffeiner A, Vilusic K, Lang I, Blum K, Obermair GG, Stefan E, Engel J, and Striessnig J. RBP2 stabilizes slow Cav1.3 Ca2+ channel inactivation properties of cochlear inner hair cells. Pflugers Archiv: Eur J Psysiol. https://doi.org/10.1007/s00424-019-02338-4