AT2 and MAS (but not AT1) angiotensinergic receptors in the medial amygdaloid nucleus modulate the baroreflex activity in rats

  • Willian Costa-Ferreira
  • Lucas Gomes-de-Souza
  • Carlos C. CrestaniEmail author
Integrative physiology
Part of the following topical collections:
  1. Integrative Physiology


The medial amygdaloid nucleus (MeA) is a limbic structure that has been demonstrated to be part of the central circuitry regulating baroreflex function. However, the local neurochemical mechanisms involved in baroreflex control by this forebrain structure is poorly understood. Thus, in the present study, we investigated the specific role of AT1, AT2, and MAS angiotensinergic receptors within the MeA in baroreflex responses in unanesthetized rats. For this, the baroreflex function was assessed using both the pharmacological approach via intravenous infusion of vasoactive agents and the sequence analysis technique. Using the pharmacological approach, we observed that bilateral microinjection of the selective AT2 receptor antagonist PD123319 into the MeA increased the tachycardia evoked by blood pressure decrease, but without affecting the reflex bradycardia caused by blood pressure increase. Besides, bilateral microinjection of the selective MAS receptor antagonist A-779 decreased both tachycardic and bradycardic responses of the baroreflex. The sequence analysis technique indicated that PD123319 into the MeA increased baroreflex effectiveness index while A-779 had an opposite effect. Treatment of the MeA with the selective AT1 receptor antagonist losartan did not affect baroreflex function assessed by either the pharmacological approach or sequence analysis technique. Overall, these findings provide evidence that MAS receptor within the MeA plays a facilitatory role in baroreflex function, whereas local AT2 receptor inhibits cardiac baroreflex responses. Results also indicate that AT1 receptor within the MeA is not involved in the control of baroreflex function.


Amygdala Losartan PD123319 A-779 AT2 receptor MAS receptor Blood pressure Heart rate 


Author contributions

WCF, LGS, and CCC contributed to the conception and design of the work. WCF and LGS contributed to the acquisition and analysis, whereas all authors contributed to data interpretation. WCF and CCC drafted the manuscript. LGS critically revised the manuscript and CCC approved the final version to be published.

Funding information

This work was supported by FAPESP (grant nos. 2015/05922-9 and 2017/19249-0, and PhD fellowship to WCF process no. 2016/05218-2), CNPq (grant no. 456405/2014-3), and Scientific Support and Development Program of School of Pharmaceutical Sciences (UNESP). CCC is a CNPq research fellow (process nos. 305583/2015-8 and 304108/2018-9).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Pharmacology, School of Pharmaceutical SciencesSão Paulo State University (UNESP)AraraquaraBrazil
  2. 2.Joint UFSCar-UNESP Graduate Program in Physiological SciencesSão CarlosBrazil

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