Robustness of the non-neuronal cholinergic system in rat large intestine against luminal challenges

  • Sandra Bader
  • Stefanie Gerbig
  • Bernhard Spengler
  • Andreas Schwiertz
  • Gerhard Breves
  • Martin DienerEmail author
Organ physiology
Part of the following topical collections:
  1. Organ Physiology


Acetylcholine and atypical esters of choline such as propionyl- and butyrylcholine are produced by the colonic epithelium and are released when epithelial receptors for short-chain fatty acids (SCFA) are stimulated by propionate. It is assumed that the SCFA used by the choline acetyltransferase (ChAT), the central enzyme for the production of these choline esters, originate from the colonic lumen, where they are synthesized during the bacterial fermentation of carbohydrates. Therefore, it seemed to be of interest to study whether the non-neuronal cholinergic system in the colonic epithelium is affected by maneuvers intended to stimulate or to inhibit colonic fermentation by changing the intestinal microbiota. In two series of experiments, rats were either fed with a high fiber diet (15.5% (w/v) crude fibers in comparison to 4.6% (w/w) in the control diet) or treated orally with the antibiotic vancomycin. High fiber diet induced an unexpected decrease in the luminal concentration of SCFA in the colon, but an increase in the caecum, suggesting an upregulation of colonic SCFA absorption, whereas vancomycin treatment resulted in the expected strong reduction of SCFA concentration in colon and caecum. MALDI MS analysis revealed a decrease in the colonic content of propionylcholine by high fiber diet and by vancomycin. High fiber diet caused a significant downregulation of ChAT expression on protein and mRNA level. Despite a modest increase in tissue conductance during the high fiber diet, main barrier and transport properties of the epithelium such as basal short-circuit current (Isc), the flux of the paracellularly transported marker, fluorescein, or the Isc induced by epithelial acetylcholine release evoked by propionate remained unaltered. These results suggest a remarkable stability of the non-neuronal cholinergic system in colonic epithelium against changes in the luminal environment underlying its biological importance for intestinal homeostasis.


Acetylcholine Antibiotics Caecum Colon Epithelium Rat Short-chain fatty acids 



The diligent technical assistance of Mrs. Marion Burmester, Brigitta Buss, Bärbel Schmidt, and Alice Stockinger is a pleasure to acknowledge. Financial support by the Deutsche Forschungsgemeinschaft (DFG) under project Sp314/13-1 is gratefully acknowledged.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute for Veterinary Physiology and BiochemistryJustus Liebig University GiessenGiessenGermany
  2. 2.Institute of Inorganic and Analytical ChemistryJustus Liebig University GiessenGiessenGermany
  3. 3.MVZ Institute of MicroecologyHerbornGermany
  4. 4.Department of PhysiologyUniversity of Veterinary Medicine Hannover, FoundationHannoverGermany
  5. 5.Institut für Veterinär-Physiologie und –BiochemieJustus-Liebig-Universität GießenGiessenGermany

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