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Resveratrol long-term treatment differentiates INS-1E beta-cell towards improved glucose response and insulin secretion

  • Marina Casimir
  • Gaelle Chaffard
  • Pierre Maechler
Molecular and cellular mechanisms of disease
  • 23 Downloads
Part of the following topical collections:
  1. Molecular and cellular mechanisms of disease

Abstract

The clonal INS-1E beta-cell line has proven to be instrumental for numerous studies investigating the mechanisms of glucose-stimulated insulin secretion. The composition of its culture medium has not changed over the years, although some compounds have been recently highlighted for their effects on tissue differentiation. The present study investigated the effects of long-term treatment of INS-1E cells with 1 μM resveratrol on glucose-stimulated insulin secretion, testing an extended glucose dose response. The data demonstrate that chronic exposure to low-dose resveratrol expands the range of the glucose dose response of INS-1E cells beyond 15 mM glucose. We also assessed whether such beneficial effects could be retained after resveratrol withdrawal from the culture medium. This was not the case as INS-1E cells deprived of resveratrol returned to the phenotype of naïve cells, i.e., exhibiting a plateau phase at 15 mM glucose. Of note, although resveratrol has antioxidant properties, it cannot substitute for β-mercaptoethanol normally present in the medium of INS-1E cells as a reducing agent. In conclusion, the addition of resveratrol as a standard component of the culture medium of INS-1E cells improves glucose-stimulated insulin secretion.

Keywords

Pancreatic beta-cell Insulin secretion Resveratrol INS-1E cells 

Notes

Acknowledgements

The authors thank Thierry Brun and Laurène Vetterli for fruitful discussions and Clarissa Bartley for analysis of AMPK.

Funding information

This work was supported by the State of Geneva and the Swiss National Science Foundation [146984 and 166625 to P.M.].

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

424_2018_2215_MOESM1_ESM.docx (15 kb)
ESM 1 (DOCX 15 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Cell Physiology and Metabolism, Faculty of MedicineUniversity of Geneva Medical CentreGeneva 4Switzerland

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