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Pflügers Archiv - European Journal of Physiology

, Volume 470, Issue 9, pp 1325–1333 | Cite as

Alterations of ATP-sensitive K+ channels in human umbilical arterial smooth muscle during gestational diabetes mellitus

  • Hongliang Li
  • Sung Eun Shin
  • Mi Seon Seo
  • Jin Ryeol An
  • Kwon-Soo Ha
  • Eun-Taek Han
  • Seok-Ho Hong
  • Jeeyoung Kim
  • Mi-Jin Yim
  • Jeong Min Lee
  • Tae Gyu An
  • Jihan Jeon
  • Se Jin Lee
  • Sung Hun NaEmail author
  • Won Sun ParkEmail author
Ion channels, receptors and transporters
Part of the following topical collections:
  1. Topical Collection: Ion channels, receptors and transporters

Abstract

We investigated the alterations of ATP-sensitive K+ (KATP) channels in human umbilical arterial smooth muscle cells during gestational diabetes mellitus (GDM). The amplitude of the KATP current induced by application of the KATP channel opener pinacidil (10 μM) was reduced in the GDM group than in the control group. Pinacidil-induced vasorelaxation was also predominant in the normal group compared with the GDM group. Reverse transcription polymerase chain reaction and Western blot analysis suggested that the expression of KATP channel subunits such as Kir6.1, Kir6.2, and SUR2B were decreased in the GDM group relative to the normal group. The application of forskolin and adenosine, which activates protein kinase A (PKA) and thereby KATP channels, elicited KATP current in both the normal and GDM groups. However, the current amplitudes were not different between the normal and GDM groups. In addition, the expression levels of PKA subunits were not altered between the two groups. These results suggest that the reduction of KATP current and KATP channel-induced vasorelaxation are due to the decreased expression of KATP channels, not to the impairment of KATP-related signaling pathways.

Keywords

ATP-sensitive K+ channels Umbilical artery Gestational diabetes mellitus Protein kinase A 

Notes

Sources of funding

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Education: 2016-R1D1A3B03930169, 2017-R1D1A1B03028467) (Ministry of Science, ICT and Future Planning: 2015-R1A4A1038666).

Compliance with ethical standards

Conflict of interest statement

The authors declare that they have no conflicts of interest.

Supplementary material

424_2018_2154_MOESM1_ESM.pptx (661 kb)
Figure S1 (PPTX 661 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hongliang Li
    • 1
  • Sung Eun Shin
    • 1
  • Mi Seon Seo
    • 1
  • Jin Ryeol An
    • 1
  • Kwon-Soo Ha
    • 2
  • Eun-Taek Han
    • 3
  • Seok-Ho Hong
    • 4
  • Jeeyoung Kim
    • 4
  • Mi-Jin Yim
    • 5
  • Jeong Min Lee
    • 5
  • Tae Gyu An
    • 6
  • Jihan Jeon
    • 6
  • Se Jin Lee
    • 6
  • Sung Hun Na
    • 6
    Email author
  • Won Sun Park
    • 1
    Email author
  1. 1.Department of PhysiologyKangwon National University School of MedicineChuncheonSouth Korea
  2. 2.Department of Molecular and Cellular BiochemistryKangwon National University School of MedicineChuncheonSouth Korea
  3. 3.Department of Medical Environmental Biology and Tropical MedicineKangwon National University School of MedicineChuncheonSouth Korea
  4. 4.Department of Internal MedicineKangwon National University School of MedicineChuncheonSouth Korea
  5. 5.Department of Applied ResearchNational Marine Biodiversity Institute of KoreaSeocheonSouth Korea
  6. 6.Department of Obstetrics and Gynecology, Kangwon National University HospitalKangwon National University School of MedicineChuncheonSouth Korea

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