Pflügers Archiv - European Journal of Physiology

, Volume 455, Issue 3, pp 555–562

Muscle transcriptome adaptations with mild eccentric ergometer exercise

  • Stephan Klossner
  • Christoph Däpp
  • Silvia Schmutz
  • Michael Vogt
  • Hans Hoppeler
  • Martin Flück
Skeletal Muscle

DOI: 10.1007/s00424-007-0303-6

Cite this article as:
Klossner, S., Däpp, C., Schmutz, S. et al. Pflugers Arch - Eur J Physiol (2007) 455: 555. doi:10.1007/s00424-007-0303-6

Abstract

The muscle has a wide range of possibilities to adapt its phenotype. Repetitive submaximal concentric exercise (i.e., shortening contractions) mainly leads to adaptations of muscle oxidative metabolism and endurance while eccentric exercise (i.e., lengthening contractions) results in muscle growth and gain of muscle strength. Modified gene expression is believed to mediate these exercise-specific muscle adjustments. In the present study, early alterations of the gene expression signature were monitored by a muscle-specific microarray. Transcript profiling was performed on muscle biopsies of vastus lateralis obtained from six male subjects before and in a 24-h time course after a single bout of mild eccentric ergometer exercise. The eccentric exercise consisted of 15 min of eccentric cycling at 50% of the individual maximal concentric power output leading to muscle soreness (5.9 on a 0–10 visual analogue scale) and limited muscle damage (1.7-fold elevated creatine kinase activity). Muscle impairment was highlighted by a transient reduction in jumping height after the eccentric exercise. On the gene expression level, we observed a general early downregulation of detected transcripts, followed by a slow recovery close to the control values within the first 24 h post exercise. Only very few regulatory factors were increased. This expression signature is different from the signature of a previously published metabolic response after an intensive endurance-type concentric exercise as well as after maximal eccentric exercise. This is the first description of the time course of changes in gene expression as a consequence of a mild eccentric stimulus.

Keywords

Human Skeletal muscle Eccentric exercise Gene expression Microarray Muscle damage 

Supplementary material

424_2007_303_MOESM1_ESM.xls (24 kb)
Supplemental Table 1Comparison of gene transcript signature after eccentric and concentric exercise. To determine which mRNA signals were significantly different (p ≤ 0.05, normal gene name) or showed a tendency (0.05 < p ≤ 0.10, italic gene name) to change throughout the time course, a Friedman ANOVA with repeated measurements was used. Significantly downregulated gene transcripts (Wilcoxon test, p ≤ 0.05) are indicated in gray and significantly upregulated gene transcripts in black; blank field: gene transcript is not detected (TIF 25 kb)
424_2007_303_MOESM2_ESM.xls (28 kb)
Supplemental Table 2Effect of an eccentric single bout on the gene transcript signature with the L2 regression analysis. To determine which mRNA signals have significantly changed (p ≤ 0.05) throughout the time course, a L2 regression permutation analysis was used. Significantly downregulated gene transcripts are indicated in gray and significant upregulated gene transcripts in black; blank field: gene transcript is not detected, v: validation of Friedman ANOVA (TIF 28 kb)

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Stephan Klossner
    • 1
  • Christoph Däpp
    • 1
  • Silvia Schmutz
    • 1
  • Michael Vogt
    • 1
  • Hans Hoppeler
    • 1
  • Martin Flück
    • 1
    • 2
  1. 1.Institute of AnatomyUniversity of BerneBerneSwitzerland
  2. 2.Institute for Biophysical and Clinical Research into Human MovementManchester Metropolitan UniversityManchesterUK

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