Pflügers Archiv - European Journal of Physiology

, Volume 455, Issue 2, pp 283–296 | Cite as

Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells

  • Yeon Jeong Kim
  • Ji Min Yu
  • Hye Joon Joo
  • Hoe Kyu Kim
  • Hyun Hwa Cho
  • Yong Chan Bae
  • Jin Sup JungEmail author
Cell and Molecular Physiology


CD9 belongs to the tetraspanin family and is involved in cell motility, osteoclastogenesis, metastasis, neurite outgrowth, myotube formation, and sperm–egg fusion. CD9 also promotes juxtacrine signaling involved in proliferation and attachment. Varying degrees of CD9 expression have been found in human mesenchymal stem cells. In this study, we determined the functional roles of CD9 in human adipose-derived mesenchymal stem cells (hASCs). The CD9 expression in hASCs was down-regulated during culture expansion. A colony-forming unit assay revealed that the clonal expandability of hASCs was directly correlated with the CD9 expression level of the colony. The CD9(high) cells exhibited an increased ability to proliferate, increased cell adhesiveness, and better in vitro tube formation than the CD9(low) cells. The cellular proliferation and attachment of the CD9(high) cells were inhibited upon treatment with a blocking antibody against CD9 and the transduction of a CD9 miRNA lentivirus. The CD9(high) cells showed higher NF-κB promoter activity and higher levels of intercellular adhesion molecule 1 than the CD9(low) cells. Reverse transcription-polymerase chain reaction analysis revealed higher endothelial nitric oxide synthase expression in the CD9(high) cells than in the CD9(low) cells. The engraftment and the proangiogenic action of hASCs in a murine model of hindlimb ischemia were significantly higher in the CD9(high) cells than in the CD9(low) cells. This study indicates that CD9 plays roles in cell proliferation and attachment in vitro as well as in in vivo engraftment and that it can be considered as a useful marker to predict the in vivo efficacy of hASCs.


CD9 Adipose tissue Mesenchymal stem cells Proliferation Adhesion Angiogenesis 



This work was supported by a grant (S06022HTB2580A) from the Korean Food & Drug Administration.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Yeon Jeong Kim
    • 1
  • Ji Min Yu
    • 1
  • Hye Joon Joo
    • 1
  • Hoe Kyu Kim
    • 1
  • Hyun Hwa Cho
    • 1
  • Yong Chan Bae
    • 2
  • Jin Sup Jung
    • 1
    • 3
    Email author
  1. 1.Department of Physiology, School of MedicinePusan National UniversityPusanSouth Korea
  2. 2.Department of Plastic Surgery, School of MedicinePusan National UniversityPusanSouth Korea
  3. 3.Medical Research InstitutePusan National UniversityPusanSouth Korea

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