Pflügers Archiv - European Journal of Physiology

, Volume 454, Issue 6, pp 989–998 | Cite as

SLC26A7 Can function as a chloride-loading mechanism in parietal cells

  • Ortrud Kosiek
  • Stephanie M. Busque
  • Michael Föller
  • Nikolay Shcheynikov
  • Philipp Kirchhoff
  • Markus Bleich
  • Shmuel Muallem
  • John P. Geibel
Gastrointestinal Function


To date three potential candidates for parietal cell basolateral Cl entry have been described: the highly 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS)-sensitive \( {{\text{Cl}}^{{\text{ - }}} } \mathord{\left/ {\vphantom {{{\text{Cl}}^{{\text{ - }}} } {{\text{HCO}}_{{\text{3}}} ^{ - } }}} \right. \kern-\nulldelimiterspace} {{\text{HCO}}_{{\text{3}}} ^{ - } } \) exchanger AE2, the \( {\text{HCO}}_{{\text{3}}} ^{ - } \) and lowly DIDS-sensitive SLC26A7 protein, and the Na+-2ClK+ cotransporter (NKCC1). In this study we investigate the contribution of these pathways to secretagogue stimulated acid secretion. Individually hand-dissected rat gastric glands were microfluorimetrically monitored for Cl influx and pHi changes. Transporter activity was determined by varying ion content and through the use of pharmacological inhibitors. Expression of SLC26A7 in rat parietal cells was shown by immunohistochemistry and Western blot. SLC26A7 was inhibited by 5-Nitro-2-(3-phenylpropyl-amino)benzoic acid (NPPB) (100 μM) in the Xenopus laevis oocyte expression system. Cl influx in parietal cells was enhanced by histamine, depended partially on endogenous \( {\text{HCO}}_{{\text{3}}} ^{ - } \) synthesis and completely on extracellular Na+. Removal and subsequent readdition of Cl revealed a low and a high DIDS-sensitive \( {\text{HCO}}_{{\text{3}}} ^{ - } \) extrusion system contributing to Cl uptake. At acidic pHi, however, H+ extrusion via the H+,K+-ATPase depending on Cl uptake was abolished only in the presence of 100 μM (NPPB) and at high (250 μM) DIDS concentration. There was no effect of the NKCC inhibitor bumetanide on stimulated H+ extrusion. These results would be compatible with SLC26A7 as a Cl uptake system under histamine stimulation.


Acid secretion Stomach pH Measurements Histamine Rat 


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Ortrud Kosiek
    • 1
    • 2
  • Stephanie M. Busque
    • 1
    • 5
  • Michael Föller
    • 1
  • Nikolay Shcheynikov
    • 4
  • Philipp Kirchhoff
    • 1
    • 3
  • Markus Bleich
    • 2
  • Shmuel Muallem
    • 4
  • John P. Geibel
    • 1
    • 5
  1. 1.Department of SurgeryYale University School of MedicineNew HavenUSA
  2. 2.Institute of PhysiologyChristian-Albrechts-UniversityKielGermany
  3. 3.Department of Visceral and Transplantation SurgeryUniversity of ZuerichZurichSwitzerland
  4. 4.Department of PhysiologyUniversity of Texas Southwestern Medical CenterDallasUSA
  5. 5.Department of Cellular and Molecular PhysiologyYale University School of MedicineNew HavenUSA

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