Stimulation of aquaporin-5 and transepithelial water permeability in human airway epithelium by hyperosmotic stress

  • Peter Steen Pedersen
  • Thomas Hartig Braunstein
  • Anders Jørgensen
  • Per Leganger Larsen
  • Niels-Henrik Holstein-Rathlou
  • Ole Frederiksen
Cell and Molecular Physiology

Abstract

Osmotic water permeability (Pf) was measured in spheroid-shaped human nasal airway epithelial explants pre-exposed to increasing levels of hyperosmotic stress. The fluid-filled spheroids, derived from nasal polyps, were lined by a single cell layer with the ciliated apical cell membrane facing the outside. The Pf was determined from diameter changes of the spheroids in response to changes in bathing medium osmolarity forth and back between 300 and 225 mOsm·l−1. Continuous diameter measurements also allowed determination of spontaneous fluid absorption. Hyperosmotic pretreatment (increase from 300 up to 600 mOsm·l−1) caused a time- and osmolarity-dependent increase (up to ∼1.5 times) in epithelial Pf which was of similar magnitude in cystic fibrosis (CF) and non-CF spheroids. The effect saturated at ∼450 mOsm·l−1 and at ∼24 h. Expression of aquaporin-5 (AQP5), studied by immunofluorescence and confocal microscopy, showed an increase in parallel with the increase in Pf following hyperosmotic stress. The AQP5 was localized both in cytoplasmic vesicles and in apical cell membranes. Spontaneous fluid absorption rates were equal in CF and non-CF spheroids and were not significantly influenced by hyperosmotic stress. The results suggest that hyperosmotic stress is an important activator of AQP-5 in human airway epithelium, leading to significantly increased transepithelial water permeability.

Keywords

Human airway epithelia Epithelial spheroids Cystic fibrosis Water permeability Fluid absorption Aquaporin-5 Hyperosmotic stress 

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Peter Steen Pedersen
    • 1
    • 2
  • Thomas Hartig Braunstein
    • 2
  • Anders Jørgensen
    • 2
  • Per Leganger Larsen
    • 3
  • Niels-Henrik Holstein-Rathlou
    • 2
  • Ole Frederiksen
    • 2
  1. 1.Department of Clinical Genetics, RigshospitaletUniversity of CopenhagenCopenhagenDenmark
  2. 2.Department of Medical Physiology, The Panum InstituteUniversity of CopenhagenCopenhagenDenmark
  3. 3.Department of Otolaryngology, Head and Neck Surgery, RigshospitaletUniversity of CopenhagenCopenhagenDenmark

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