Pflügers Archiv

, Volume 451, Issue 3, pp 464–473 | Cite as

Water permeability in human airway epithelium

  • Peter Steen Pedersen
  • Kristina Procida
  • Per Leganger Larsen
  • Niels-Henrik Holstein-Rathlou
  • Ole Frederiksen
Ion Channels, Transporters


Osmotic water permeability (Pf) was studied in spheroid-shaped human airway epithelia explants derived from nasal polyps by the use of a new improved tissue collection and isolation procedure. The fluid-filled spheroids were lined with a single cell layer with the ciliated apical cell membrane facing the outside. They were capable of surviving hours of experiment involving continuous superfusion of the bathing medium and changes of osmolarity. A new image analysis technique was developed for measuring the spheroid diameters, giving high time and measurement resolutions. The transepithelial Pf, determined by the changes of the apical solution osmolarity, was not influenced by the presence of glucose, Na+, or Na+/glucose-cotransport inhibitors in the bath, but was sensitive to the aquaporin (AQP) inhibitor HgCl2. The measured Pf levels and the values of activation energy were in the range of those seen in AQP-associated water transport. Together, these results indicate the presence of an AQP in the apical membrane of the spheroids. Notably, identical values for Pf were found in CF and non-CF airway preparations, as was the case also for the calculated spontaneous fluid absorption rates.


Human airway epithelia Spheroids Cystic fibrosis Water permeability Fluid absorption Aquaporin 


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Peter Steen Pedersen
    • 1
    • 2
  • Kristina Procida
    • 2
  • Per Leganger Larsen
    • 3
  • Niels-Henrik Holstein-Rathlou
    • 2
  • Ole Frederiksen
    • 2
  1. 1.Department of Clinical Genetics, RigshospitaletUniversity of CopenhagenCopenhagenDenmark
  2. 2.Department of Medical PhysiologyThe Panum Institute, University of CopenhagenCopenhagenDenmark
  3. 3.Department of Otolaryngology, Head and Neck Surgery, RigshospitaletUniversity of CopenhagenCopenhagenDenmark

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