Pflügers Archiv

, Volume 450, Issue 4, pp 209–216 | Cite as

Resting metabolism of mouse papillary muscle

Cardiovascular System


The aims of this study were to measure the resting metabolic rate of isolated mouse papillary muscles and to determine whether diffusive O2 supply is adequate to support the resting metabolism. Resting metabolism of left ventricular papillary muscles was measured in vitro (27°C) using the myothermic technique. The rate of resting metabolism declined exponentially with time towards a steady value, with a time constant of 18±2 min (n=13). There was no alteration in isometric force output during this time. The magnitude of the resting metabolism, which depended inversely on muscle mass, more than doubled following a change in substrate from glucose to pyruvate and was increased 2.5-fold when the osmolarity of the bathing solution was increased by addition of 300 mM sucrose. Addition of 30 mM 2, 3-butanedione monoxime affected neither the time course of the decline in metabolic rate nor the eventual steady value. Analysis of the diffusive oxygen supply to the isolated preparation indicated that small papillary muscles (mass <1 mg), which have a very high resting metabolic rate early in an experiment, are unlikely to be adequately oxygenated.


Metabolic Rate Papillary Muscle Contractile Activity Critical Radius Sarcomere Length 



This work was supported by the Heart Foundation Research Centre, Griffith University.


  1. 1.
    Backx PH, Gao WD, Azan-Backx MD, Marban E (1994) Mechanism of force inhibition by 2,3-butanedione monoxime in rat cardiac muscle: roles of [Ca2+]i and cross-bridge kinetics. J Physiol (Lond) 476:487–500Google Scholar
  2. 2.
    Barclay CJ, Arnold PD, Gibbs CL (1995) Fatigue and heat production in repeated contractions of mouse skeletal muscle. J Physiol (Lond) 488:741–752Google Scholar
  3. 3.
    Barclay CJ, Widén C, Mellors LJ (2003) Initial mechanical efficiency of isolated cardiac muscle. J Exp Biol 206:2725–2732CrossRefPubMedGoogle Scholar
  4. 4.
    Baxi J, Barclay CJ, Gibbs CL (2000) Energetics of rat papillary muscle during contractions with sinusoidal length changes. Am J Physiol 278:H1545–H1554Google Scholar
  5. 5.
    Biron R, Burger A, Chinet A, Clausen T, Dubois-Ferriere R (1979) Thyroid hormones and the energetics of active sodium-potassium transport in mammalian skeletal muscles. J Physiol (Lond) 297:47–60Google Scholar
  6. 6.
    Bluhm WF, Kranias EG, Dillmann WH, Meyer M (2000) Phospholamban: a major determinant of the cardiac force-frequency relationship. Am J Physiol 278:H249–H255Google Scholar
  7. 7.
    Bolli R, Marban E (1999) Molecular and cellular mechanisms of myocardial stunning. Physiol Rev 79:609–634PubMedGoogle Scholar
  8. 8.
    Daut J, Elzinga G (1988) Heat production of quiescent ventricular trabeculae isolated from guinea-pig heart. J Physiol (Lond) 398:259–275Google Scholar
  9. 9.
    Dietrich DL, Elzinga G (1993) Heat produced by rabbit papillary muscle during anoxia and reoxygenation. Circ Res 73:1177–1187PubMedGoogle Scholar
  10. 10.
    Donald TC, Reeves DN, Reeves RC, Walker AA, Hefner LL (1980) Effect of damaged ends in papillary muscle preparations. Am J Physiol 238:H14–H23PubMedGoogle Scholar
  11. 11.
    Ford LE, Huxley AF, Simmons RM (1977) Tension responses to sudden length change in stimulated frog muscle fibres near slack length. J Physiol (Lond) 269:441–515Google Scholar
  12. 12.
    Gibbs CL, Loiselle DS (2001) Cardiac basal metabolism. Jpn J Physiol 51:399–426CrossRefPubMedGoogle Scholar
  13. 13.
    Gibbs CL, Mommaerts WF, Ricchiuti NV (1967) Energetics of cardiac contractions. J Physiol (Lond) 191:25–46Google Scholar
  14. 14.
    Gustafson LA, Van Beek JH (2000) Measurement of the activation time of oxidative phosphorylation in isolated mouse hearts. Am J Physiol 279:H3118–H3123Google Scholar
  15. 15.
    He H, Giordano FJ, Hilal-Dandan R, Choi DJ, Rockman HA, McDonough PM, Bluhm WF, Meyer M, Sayen MR, Swanson E, Dillmann WH (1997) Overexpression of the rat sarcoplasmic reticulum Ca2+ ATPase gene in the heart of transgenic mice accelerates calcium transients and cardiac relaxation. J Clin Invest 100:380–389PubMedGoogle Scholar
  16. 16.
    Hill AV (1928) The diffusion of oxygen and lactic acid through tissues. Proc R Soc B 104:39–96Google Scholar
  17. 17.
    Hill AV (1965) Trails and trials in physiology. Arnold, LondonGoogle Scholar
  18. 18.
    Hisano R, Cooper G (1987) Correlation of force-length area with oxygen consumption in ferret papillary muscle. Circ Res 61:318–328PubMedGoogle Scholar
  19. 19.
    Josephson R, Stokes D (1994) Contractile properties of a high-frequency muscle from a crustacean—mechanical power output. J Exp Biol 187:295–303PubMedGoogle Scholar
  20. 20.
    Kiriazis H, Gibbs CL (1995) Papillary muscles split in the presence of 2,3-butanedione monoxime have normal energetic and mechanical properties. Am J Physiol 269:H1685–H1694PubMedGoogle Scholar
  21. 21.
    Kohler I, Meier R, Busato A, Neiger-Aeschbacher G, Schatzmann U (1999) Is carbon dioxide (CO2) a useful short acting anaesthetic for small laboratory animals? Lab Anim 33:155–161PubMedGoogle Scholar
  22. 22.
    Kretzschmar KM, Wilkie DR (1972) A new method for absolute heat measurement, utilizing the Peltier effect. J Physiol (Lond) 224:18P–21PGoogle Scholar
  23. 23.
    Loiselle D (1985) A theoretical analysis of the rate of resting metabolism of isolated papillary muscle. Adv Myocardiol 6:205–216PubMedGoogle Scholar
  24. 24.
    Loiselle DS (1985) The effect of temperature on the basal metabolism of cardiac muscle. Pflugers Arch 405:163–169CrossRefPubMedGoogle Scholar
  25. 25.
    Loiselle DS (1985) The rate of resting heat production of rat papillary muscle. Pflugers Arch 405:155–162CrossRefPubMedGoogle Scholar
  26. 26.
    Loiselle DS (1987) The effect of myoglobin-facilitated oxygen transport on the basal metabolism of papillary muscle. Biophys J 51:905–913PubMedGoogle Scholar
  27. 27.
    Loiselle DS, Gibbs CL (1979) Species differences in cardiac energetics. Am J Physiol 237:H90–H98PubMedGoogle Scholar
  28. 28.
    Loiselle DS, Gibbs CL (1983) Factors affecting the metabolism of resting rabbit papillary muscle. Pflugers Arch 396:285–291CrossRefPubMedGoogle Scholar
  29. 29.
    Loiselle DS, Stienen GJ, van Hardeveld C, van der Meulen ET, Zahalak GI, Daut J, Elzinga G (1996) The effect of hyperosmolality on the rate of heat production of quiescent trabeculae isolated from the rat heart. J Gen Physiol 108:497–514CrossRefPubMedGoogle Scholar
  30. 30.
    Mahler M, Louy C, Homsher E, Peskoff A (1985) Reappraisal of diffusion, solubility, and consumption of oxygen in frog skeletal muscle, with applications to muscle energy balance. J Gen Physiol 86:105–134CrossRefPubMedGoogle Scholar
  31. 31.
    Meyer M, Bluhm WF, He H, Post SR, Giordano FJ, Lew WY, Dillmann WH (1999) Phospholamban-to-SERCA2 ratio controls the force-frequency relationship. Am J Physiol 276:H779–H785PubMedGoogle Scholar
  32. 32.
    Mulieri LA, Luhr G, Trefry J, Alpert NR (1977) Metal-film thermopiles for use with rabbit right ventricular papillary muscles. Am J Physiol 233:C146–C156PubMedGoogle Scholar
  33. 33.
    Redel A, Baumgartner W, Golenhofen K, Drenckhahn D, Golenhofen N (2002) Mechanical activity and force-frequency relationship of isolated mouse papillary muscle: effects of extracellular calcium concentration, temperature and contraction type. Pflugers Arch 445:297–304CrossRefPubMedGoogle Scholar
  34. 34.
    Stull LB, Leppo MK, Marban E, Janssen PM (2002) Physiological determinants of contractile force generation and calcium handling in mouse myocardium. J Mol Cell Cardiol 34:1367–1376CrossRefPubMedGoogle Scholar
  35. 35.
    Stuyvers BD, McCulloch AD, Guo J, Duff HJ, ter Keurs HE (2002) Effect of stimulation rate, sarcomere length and Ca2+ on force generation by mouse cardiac muscle. J Physiol (Lond) 544:817–830CrossRefGoogle Scholar
  36. 36.
    Wang JF, Yan X, Min J, Sullivan MF, Hampton TG, Morgan JP (2002) Cocaine downregulates cardiac SERCA2a and depresses myocardial function in the murine model. Can J Physiol Pharmacol 80:1015–1021CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.School of Physiotherapy and Exercise ScienceGriffith UniversityGold CoastAustralia

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