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Pflügers Archiv

, Volume 448, Issue 2, pp 161–174 | Cite as

Modeling of stimulation–secretion coupling in a chromaffin cell

  • A. WarashinaEmail author
  • T. Ogura
Cell and Molecular Physiology

Abstract

We constructed a chromaffin cell model for analysis of stimulation–secretion coupling in computer simulation studies. The model includes mechanisms involved in the excitatory synapse, voltage-dependent Na+, K+ and Ca2+ channels, Ca2+-activated K+ channels (SK type), buffered Ca2+ diffusion, Ca2+ extrusion, fluorescent Ca2+ indicators and Ca2+-triggered exocytosis. Calculations of the modeled mechanisms were carried out using the NEURON simulation environment (Hines and Carnevale, Neural Computation 9:1179–1209, 1997). A set of parameter values was determined so as to fit basic experimental results reported in the literature. The model was also applied to simulate our experimental results obtained from chromaffin cells in the perfused rat adrenal medulla. Observed profiles of Ca2+responses induced by electrically stimulating the splanchnic nerve with various frequencies (1–50 Hz) were adequately simulated with minor readjustments of parameter values for Ca2+influx and extrusion. Secretory responses measured at the same time as the Ca2+responses were also simulated with consideration of a time constant to detect catecholamines in the experiment. Similarly, model simulations reproduced both Ca2+responses and secretory responses evoked by elevations of the extracellular K+ concentration for different periods. The results suggest that the presented model provides a useful tool for analyzing and predicting quantitative relations in various events occurring in stimulation–secretion coupling in chromaffin cells.

Keywords

Chromaffin cell model  Computer simulation Stimulation–secretion coupling  

Notes

Acknowledgement

This study was supported in part by NIH/NIDCD grants DC 05140 (TO) and P30 DC 04657 (Diego Restrepo of Univ. Colorado Health Sciences Center).

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Copyright information

© Springer-Verlag  2004

Authors and Affiliations

  1. 1.Division of Cell PhysiologyNiigata University Graduate School of Medical and Dental SciencesNiigata Japan
  2. 2.Department of Biomedical SciencesColorado State UniversityFort CollinsUSA
  3. 3.Department of Cellular and Structural BiologyUniversity of Colorado Health Sciences CenterDenverUSA

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