Pflügers Archiv

, Volume 447, Issue 5, pp 760–762 | Cite as

The acetyl-CoA transporter family SLC33

  • Yoshio Hirabayashi
  • Akiko Kanamori
  • Kazuko H. Nomura
  • Kazuya Nomura
The ABC of Solute Carriers Guest Editor: Matthias A. Hediger

Abstract

The acetyl-CoA (Ac-CoA) transporter (AT-1) is a multiple transmembrane protein in the endoplasmic reticulum. Ac-CoA is transported to the lumen of the Golgi apparatus, where it serves as the substrate of acetyltransferases that modify the sialyl residues of gangliosides and glycoproteins. The AT-1 gene, originally named ACATN (acetyl-CoA transporter), was cloned from human melanoma cells. Although homologs of this family of proteins have been identified in lower organisms, such as Escherichia coli, Drosophila melanogaster, and Caenorhabditis. elegans, currently only one member of this SLC33A1 family has been identified in humans. Thus, SLC33A1 proteins should be re-named ACATN1 or AT-1. Although acetylated gangliosides show a highly tissue-specific distribution, AT-1 is ubiquitously expressed. Phylogenetically, the AT-1 gene is highly conserved, suggesting that it is particularly significant. The precise physiological roles of this transporter protein, however, remain to be elucidated.

Keywords

Acetyl-CoA transporter Endoplasmic reticulum 

References

  1. 1.
    Butor C, Diaz S, Varki A (1993) High level O-acetylation of sialic acids on N-linked oligosaccharides of rat liver membranes. Differential subcellular distribution of 7- and 9-O-acetyl groups and of enzymes involved in their regulation. J Biol Chem 268:10197–10206PubMedGoogle Scholar
  2. 2.
    Varki A, Hooshmand F, Diaz S, Varki NM, Hedrick SM (1991) Developmental abnormalities in transgenic mice expressing a sialic acid-specific 9-O-acetylesterase. Cell 65:65–74PubMedGoogle Scholar
  3. 3.
    Malisan F, Franchi L, Tomassini B, Ventura N, Condo I, Rippo MR, Rufini A, Liberati L, Nachtigall C, Kniep B, Testi R (2002) Acetylation suppresses the proapoptotic activity of GD3 ganglioside. J Exp Med 196:1535–1541CrossRefPubMedGoogle Scholar
  4. 4.
    Varki A, Diaz S (1985) The transport and utilization of acetyl coenzyme A by rat liver Golgi vesicles. O-acetylated sialic acids are a major product. J Biol Chem 260:6600–6608PubMedGoogle Scholar
  5. 5.
    Kanamori A, Nakayama J, Fukuda MN, Stallcup WB, Sasaki K, Fukuda M, Hirabayashi Y (1997) Expression cloning and characterization of a cDNA encoding a novel membrane protein required for the formation of O-acetylated ganglioside: a putative acetyl-CoA transporter. Proc Natl Acad Sci USA 94:2897–2902CrossRefPubMedGoogle Scholar
  6. 6.
    Bora RS, Kanamori A, Hirabayashi Y (1999) Cloning and characterization of a putative mouse acetyl-CoA transporter cDNA. Gene 238:455–462CrossRefPubMedGoogle Scholar
  7. 7.
    Bora RS, Ichikawa S, Kanamori A, Hirabayashi Y (2000) cDNA cloning of putative rat acetyl-CoA transporter and its expression pattern in brain. Cytogenet Cell Genet 89:204–208CrossRefPubMedGoogle Scholar
  8. 8.
    Bora RS, Ichikawa S, Kanamori A, Hirabayashi Y (2000) Genomic structure and promoter analysis of putative mouse acetyl-CoA transporter gene. FEBS Lett 473:169–172CrossRefPubMedGoogle Scholar
  9. 9.
    Krogh A, Larsson B, Heijne G von, Sonnhammer EL (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305:567–580CrossRefPubMedGoogle Scholar
  10. 10.
    Bora RS, Kanamori A, Hirabayashi Y (1998) Assignment1 of a putative acetyl-CoA transporter gene (Acatn) to mouse chromosome band 3E1-E3 by in situ hybridization. Cytogenet Cell Genet 83:78–79CrossRefPubMedGoogle Scholar
  11. 11.
    Jacobs C, Huang LJ, Bartowsky E, Normark S, Park JT (1994) Bacterial cell wall recycling provides cytosolic muropeptides as effectors for beta-lactamase induction. EMBO J 13:4684–4694PubMedGoogle Scholar
  12. 12.
    Cheng Q, Park JT (2002) Substrate specificity of the AmpG permease required for recycling of cell wall anhydro-muropeptides. J Bacteriol 184:6434–6436CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag  2004

Authors and Affiliations

  • Yoshio Hirabayashi
    • 1
  • Akiko Kanamori
    • 1
  • Kazuko H. Nomura
    • 2
  • Kazuya Nomura
    • 2
  1. 1.Neuronal Circuit Mechanisms Research GroupRIKEN Brain Science InstituteSaitamaJapan
  2. 2.Department of Biological Sciences, Faculty of SciencesKyushu University Graduate SchoolFukuoka-shiJapan

Personalised recommendations