Pflügers Archiv

, Volume 445, Issue 5, pp 601–606

Regulation of KCNE1-dependent K+ current by the serum and glucocorticoid-inducible kinase (SGK) isoforms

  • Hamdy M. Embark
  • Christoph Böhmer
  • Volker Vallon
  • Friedrich Luft
  • Florian Lang
Ion Channels, Transporters

DOI: 10.1007/s00424-002-0982-y

Cite this article as:
Embark, H.M., Böhmer, C., Vallon, V. et al. Pflugers Arch - Eur J Physiol (2003) 445: 601. doi:10.1007/s00424-002-0982-y

Abstract

The slowly activating K+ channel subunit KCNE1 is expressed in a variety of tissues including proximal renal tubules, cardiac myocytes and stria vascularis of inner ear. The present study has been performed to explore whether the serum- and glucocorticoid-inducible kinase family members SGK1, SGK2, or SGK3 and/or protein kinase B (PKB) influence K+ channel activity in Xenopus oocytes expressing KCNE1. cRNA encoding KCNE1 was injected with or without cRNA encoding wild-type SGK1, constitutively active S422DSGK1, inactive K127 NSGK1, wild-type SGK2, wild-type SGK3 or constitutively active T308D,S473DPKB. In oocytes injected with KCNE1 cRNA but not in water-injected oocytes a depolarization from −80 mV to −10 mV led to the appearance of a slowly activating K+ current. Coexpression of SGK1, S422DSGK1, SGK2, SGK3 or T308D,S473DPKB but not K127 NSGK1 significantly stimulated KCNE1-induced current. The effect did not depend on Na+/K+-ATPase activity. KCNE1-induced current was markedly upregulated by coexpression of KCNQ1 and further increased by additional expression of S422DSGK1, SGK2, SGK3 or T308D,S473DPKB. In conclusion, all three members of the SGK family of kinases SGK1–3 and protein kinase B stimulate the slowly activating K+ channel KCNE1/KCNQ1. The kinases may thus participate in the regulation of KCNE1-dependent transport and excitability.

Keywords

Glucocorticoids Heart IGF-1 Inner ear Insulin Intestine Kidney Mineralocorticoids PKB 

Copyright information

© Springer-Verlag  2003

Authors and Affiliations

  • Hamdy M. Embark
    • 1
  • Christoph Böhmer
    • 1
  • Volker Vallon
    • 2
  • Friedrich Luft
    • 3
  • Florian Lang
    • 1
  1. 1.Department of PhysiologyUniversity of TübingenTübingenGermany
  2. 2.Department of PharmacologyUniversity of Tübingen TübingenGermany
  3. 3.Department of Internal MedicineChariteBerlinGermany

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