Langenbeck's Archives of Surgery

, Volume 395, Issue 8, pp 1147–1155 | Cite as

Microvascular response to calcium phosphate bone substitutes: an intravital microscopy analysis

  • Bernd Roetman
  • Andrej Ring
  • Stefan Langer
  • Thomas A. Schildhauer
  • Gert Muhr
  • Manfred Köller
Original Article



The purpose was to evaluate inflammatory and microcirculatory reactions after implantation of various calcium phosphate bone substitutes in an in vivo model.


Calcium phosphate-based bone substitutes were implanted in dorsal skinfold chambers of mice. Intravital fluorescence microscopy was performed to measure inflammatory and microcirculatory reactions based on functional vessel density (FVD), capillary leakage, and relative white blood cell velocity (rWBCV).


An increase of FVD was observed in all groups and the capillary leakage grew with a level of significance (p < 0.001). The fraction of rolling and sticking leukocytes (rWBCV) was highest at the beginning of the trial and decreased during the course.


There are differences in microvascular soft tissue reactions between various calcium phosphate bone substitutes, but inflammatory reactions were moderate, and the results revealed no reasons which explain the sporadic failure of the tested substances under clinical conditions.


Fluorescence microscopy Dorsal skinfold chamber Resorbable bone substitutes Inflammatory reaction Microvascular response 


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Bernd Roetman
    • 1
    • 4
  • Andrej Ring
    • 2
  • Stefan Langer
    • 2
  • Thomas A. Schildhauer
    • 3
  • Gert Muhr
    • 1
  • Manfred Köller
    • 1
  1. 1.Chirurgische Klinik und PoliklinikBerufsgenossenschaftliches Universitätsklinikum Bergmannsheil GmbHBochumGermany
  2. 2.Klinik für Plastische-, Hand- und SchwerstverbranntenchirurgieBerufsgenossenschaftliches Universitätsklinikum Bergmannsheil GmbHBochumGermany
  3. 3.Universitätsklinik für Unfallchirurgie und SporttraumatologieMedizinische Universitätsklinik, GrazGrazAustria
  4. 4.Berufsgenossenschaftliches Universitätsklinikum Bergmannsheil GmbHChirurgische Klinik und PoliklinikBochumGermany

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