Langenbeck's Archives of Surgery

, Volume 392, Issue 3, pp 285–295

Distinct effects of acute and chronic nicotine application on microvascular thrombus formation and endothelial function in male and female mice

  • Nicole Lindenblatt
  • Uwe Platz
  • Jörn Hameister
  • Ernst Klar
  • Michael D. Menger
  • Brigitte Vollmar
Original Article
  • 97 Downloads

Abstract

Background and aims

Cigarette smoking is linked to thromboembolic events; however, a relationship between nicotine exposition and thrombosis has not been established. Thus, we intended to study the effect of acute and chronic nicotine application in an in vivo mouse model.

Materials and methods

In microvessels of the dorsal skin fold chamber, light-dye-induced thrombus formation was analyzed using intravital fluorescence microscopy. Male and female C57BL/6J mice received nicotine chronically via the drinking water (100 μg/ml) for 8 weeks. An additional series of experiments was performed with acute iv nicotine treatment (3 mg/kg body weight).

Results

No significant differences in microvascular thrombus formation were detected after chronic nicotine application in male and female animals when compared with controls. Accordingly, flow cytometric analysis did not show significant effects on platelet activity. Chronic nicotine treatment resulted in a significantly reduced endothelial activation in male, but not in female mice. In contrast, acute iv application of nicotine revealed significantly shorter thrombosis times in arterioles of female mice and a significantly increased endothelial P-selectin expression in mice of both genders.

Conclusion

Chronic nicotine application does not promote microvascular thrombus formation in mice of either gender, whereas acute high-dose iv administration caused a significant increase of arteriolar thrombosis in female animals probably via a synergistic effect of increased endothelial P-selectin expression and female hormone levels. A gender-dependency of acute nicotine action can be presumed.

Keywords

Nicotine Microvascular thrombosis Dorsal skinfold chamber Endothelium Platelet 

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Nicole Lindenblatt
    • 1
    • 2
  • Uwe Platz
    • 1
  • Jörn Hameister
    • 3
  • Ernst Klar
    • 2
  • Michael D. Menger
    • 4
  • Brigitte Vollmar
    • 1
  1. 1.Institute for Experimental SurgeryUniversity of RostockRostockGermany
  2. 2.Department of General SurgeryUniversity of RostockRostockGermany
  3. 3.Institute for ToxicologyUniversity of RostockRostockGermany
  4. 4.Institute for Clinical and Experimental SurgeryUniversity of SaarlandHomburg-SaarGermany

Personalised recommendations